In-silico insights into targeting OmpA: an approach to combat multidrug resistance in .

Abstract: The rise of multidrug-resistant poses significant challenges in hospital settings. This study evaluates the antimicrobial potential of the aqueous extract of (AETC) against strain AB0014, isolated from a preterm neonate presenting sepsis. The minimum inhibitory concentration (MIC) was determined using the microdilution method.

Metabolite profiling and neuroprotective potential of Clitoria ternatea L. through in-vitro and in-vivo experimental models.

Introduction: Alzheimer's disease is a growing concern in the global population, affecting learning, memory and causes neurodegeneration. Clitoria ternatea L. (Fabaceae), is used traditionally to treat cognitive dysfunction by improving memory.

Antiviral efficacy of hexane extract of Hypericum gaitii Haines against Chikungunya and SARS-CoV-2 viruses: in vitro and in silico approaches.

Ethnopharmacological Relevance: Viral infections have become a public health concern globally. The limited efficacy of existing antivirals against emerging viruses encourages extensive research to find alternative strategies. In this scenario, various plant bioactive compounds have been reported to possess potential against a wide range of illnesses, including viral diseases.

Process optimization and partial purification of a highly catalytic xylanase from high-altitude novel Bacillus altitudinis strain for efficient production of functional prebiotic xylooligosaccharides.

This study reports the production, statistical optimization, partial purification and characterization of a highly efficient xylanase from a high-altitude soil bacterium, Bacillus altitudinis XYL17, for the enzymatic valorization of agrowaste. A two-step optimization approach combining Plackett-Burman Design (PBD) and Response Surface Methodology (RSM) enhanced xylanase production with minimal resource utilization. The partially purified enzyme exhibited optimal activity at 50-55 °C and pH 6.

Allele-specific expression in non-small cell lung cancer: A genome-wide investigation.

Several studies have suggested that allele-specific expression (ASE)plays a critical role in cancer initiation and progression. However, little is known about genome-wide ASE and monoallelic expression of genes in non-small cell lung cancer (NSCLC). We analysed 11,422 genes that had either at least two Single Nucleotide Variations (SNVs) or a high-confidence single SNV (depth ≥10) in metastatic NSCLC samples, and found that approximately 43 % (4935 genes) exhibited monoallelic expression in at least one sample.

Quantification of anthocyanins in Oryza sativa L. from Jammu and Kashmir, India by a validated high performance thin layer chromatography (HPTLC) method.

Anthocyanins are bioactive compounds with antioxidant, anti-obesity, and anti-inflammatory properties beneficial for human health. HPTLC is an effective technique that is ideal for analysing both qualitatively and quantitatively. One of the advantages of HPTLC over HPLC (High performance liquid chromatography) and LC-MS (Liquid chromatography mass spectrometry) is the capability to analyse crude samples consisting of various compounds along with the capacity to apply multiple samples and a range of standards on a single plate with enhanced accuracy and precision of quantification without constrains of time.

Novel endophytic actinomycetes species Streptomyces panacea of Panax sokpayensis produce antimicrobial compounds against multidrug resistant Staphylococcus aureus.

Endophytic actinomycetes of medicinal plants have recently been in focus for developing novel antimicrobial compounds to combat multidrug-resistant pathogens. In this study, we isolated and characterised endophytic actinomycetes of Panax sokpayensis rhizome traditionally used as medicine in Sikkim-Himalayan region and assessed their antimicrobial activity against multidrug-resistant (MDR) clinical isolates of Staphylococcus aureus. Saccharopolyspora dominated as the endophytic actinomycetes of P.

Immunomodulatory activity of bamboo biosilica from Dendrocalamus longispathus Kurz. - Metabolomics integrated network pharmacology approach.

Background: Bamboo has been used in traditional Asian medicine since ancient times. Silica is formed in the shoot internodes of various bamboo species. This bamboo-derived silica, referred to as bamboo biosilica or "banslochan" (in Sanskrit), possesses multiple health benefits.

Unveiling molecular mechanisms of iron and zinc dynamics in rice.

Iron (Fe) and zinc (Zn) are essential micronutrients critical for human health, yet their deficiencies are widespread, particularly in rice-dependent populations. Rice, a staple food for over half the global population, lacks sufficient bioavailable Fe and Zn in its grains, contributing to global malnutrition. This review delves into the molecular mechanisms governing Fe and Zn transport in rice, focusing on gene families such as IRT, YSL, ZIP, and HMA, which regulate uptake, translocation, and storage.

Toxicological evaluation of Isosecotanapartholide from Artemisia vulgaris L.: oral acute and sub-acute toxicity in BALB/c mice.

Isosecotanapartholide (ISTP) isolated from Artemisia vulgaris, having fumigant property, was tested for acute and sub-acute toxicity in BALB/c mice. The male and female BALB/c mice were given ISTP orally for 7 days at doses of 200, 800 and 1600 mg/kg body weight (bw) for the acute toxicity assay. For sub-acute toxicity test, ISTP was given orally for 14 days at doses of 50 and 500 mg/kg bw.

Corrigendum to "Threats of zoonotic pathogens in food chain: Current status and gaps in India" [Heliyon Volume 11, Issue 1, 15 January 2025, e41240].

[This corrects the article DOI: 10.1016/j.heliyon.

Endotoxin (lipopolysaccharide)-induced inflammation in albino rat and macrophages (RAW 264.7): Piper mullesua leaf extract as promising therapeutic against inflammatory pathophysiology via SOCS1 activation and phospho-NF-κB/JAK1/STAT1 inhibition.

The present investigation has been attempted for scientifically validating the traditional uses of Piper mullesua against inflammatory pathophysiology using both in vivo (albino rats) and in vitro (macrophage cells, RAW 264.7) models of inflammation caused by an endotoxin (lipopolysaccharide, LPS). Oral gavaging with PMHAE, hydroalcoholic extract of Piper mullesua leaves, dose-responsively (50, 100, or 200 mg/kg BW, 14 days) restored any alteration in the concentration of serum inflammatory cytokines, IL-6, TNF-α, IL-4, and IL-10 and decreased prostaglandin (PGE2) and nitrite count in rats injected (i.

Revolutionizing Drug Development: Harnessing Artificial Intelligence in Pharmaceutical Sciences.

The integration of artificial intelligence (AI) in pharmaceutical sciences marks a signifi-cant milestone in the field of drug discovery and development, presenting unique prospects for crea-tivity and productivity. This review article delves into the significant impact of AI on contemporary pharmaceutical practices, highlighting its incorporation in different phases of drug discovery and personalized medicine. Our goal is to offer a thorough analysis of the current landscape of AI appli-cations in the field, outline the extent of recent progress, and explore the obstacles and potential future paths for AI technologies.

Metabolomic study of Polygonum posumbu Buch. Ham. Ex D. Don integrating with anti-diabetic potential - a mechanistic approach.

The present investigation aimed to evaluate the anti-diabetic effect of the well-known food plant Polygonum posumbu along with the mechanism of combining its in-vitro, in-vivo effects and UHPLC-QTOF-MS based phytochemical profiling linked with the network pharmacology. In-vitro anti-diabetic potential of the extract was determined by testing against α-amylase and α-glucosidase enzymes. The in-vivo anti-diabetic effect of the extract was investigated in STZ-induced diabetic mice.

Investigating the impact of Terminalia chebula, an underutilized functional fruit, on oral squamous cell carcinoma: Exploring cell death mechanisms.

Ethnopharmalogical Relevance: Terminalia chebula, known for its extensive use in traditional medicinal practices among indigenous cultures, is recognized for its effectiveness in treating various oral disorders. Healers in India and China utilize the ripe fruits of T. chebula to prevent and manage conditions such as dental cavities, gingivitis, bleeding gums and stomatitis.

Therapeutics of bitter plants from Northeast region of India and their pharmacological and phytochemical perspectives.

Natural resources have been used for food and medicine since the beginning of human civilization, and they have always been a low-cost, easily accessible source for individuals. Northeast region of India (NER) represents a significant portion of India's flora and fauna. Marginality, fragility, inaccessibility, ethnicity, and cultural diversity thrived in the region, resulting in the richest reservoir of genetic variation of bioresources.

Unearthing novel and multifunctional peptides in peptidome of fermented chhurpi cheese of Indian Himalayan region.

Fermented foods of the Indian Himalaya are unexplored functional resources with high nutritional potential. Chhurpi cheese, fermented by defined native proteolytic lactic acid bacteria of Sikkim was assessed for ACE inhibitory, HOCl reducing, and MPO inhibitory, activity across varying stages of gastrointestinal (GI) digestion. The enhanced bioactivity of Lactobacillus delbrueckii WS4 chhurpi was associated with the generation of bioactive and multifunctional peptides during fermentation and GI digestion.

A Validated Method for Identification and Quantification of Anthocyanins in Different Black Rice (Oryza sativa L.) Varieties Using High-Performance Thin-Layer Chromatography (HPTLC).

Introduction: Black rice (Oryza sativa L.) has gained prominence as a functional food because of its rich content of anthocyanins and polyphenols, offering potential health benefits. However, comprehensive research addressing the diverse anthocyanin compositions in black rice cultivars remains limited.

Fermented foods affect the seasonal stability of gut bacteria in an Indian rural population.

The effect of fermented foods on healthy human gut microbiota structure and function, particularly its seasonal preference and frequent long-term consumption, has been largely uncharacterised. Here, we assess the gut microbiota and metabolite composition of 78 healthy Indian agrarian individuals who differ in the intake of fermented milk and soybean products by seasonal sampling during hot-humid summer, autumn and dry winter. Here we show that, seasonal shifts between the Prevotella- and Bifidobacterium/Ruminococcus-driven community types, or ecological states, and associated fatty acid derivatives, with a bimodal change in Bacteroidota community structure during summer, particularly in fermented milk consumers.

Mechanistic insight into neuroprotective effect of standardized ginger chemo varieties from Manipur, India in scopolamine induced learning and memory impaired mice.

Alzheimer's disease is a complex neurodegenerative disease characterized by progressive decline in cognitive function and behaviour. Ginger is the rhizome of the plant Zingiber officinale Roscoe, has been an important ingredient of many Ayurveda formulations to treat neurological disorders. The present study aims to estimate the variation of 6-gingerol content in nine different ginger samples collected from Manipur, India, investigate the neuroprotective potential of the most potent ginger sample against scopolamine-induced cognitively impaired mice, and validate the therapeutic claim by molecular docking analysis.

Threats of zoonotic pathogens in food chain: Current status and gaps in India.

Background: Public health is seriously threatened by transmission of zoonotic infection through the food chain. Factors like increasing population, deforestation, high demand for animal protein, and trade of sub-clinically infected animals are the main causes of the spread of infections from asymptomatic animals to humans. Despite several national programs like (The Clean India Mission) prevention of open defecation and water, sanitation, and hygiene (WASH), the incidence of diarrhoeal diseases remains high in India.

Capsaicin content in chillies ( L., s L., Jacq.) of Northeast India by high performance thin layer chromatography (HPTLC).

Capsaicin is the primary bioactive constituent in chillies, responsible for its incomparable pungent taste and many health advantages. In the current study, 32 samples of three different species of ( L., s L.

Relevance of Indian Traditional Herbal Brews for Gut Microbiota Balance.

The considerable changes in lifestyle patterns primarily affect the human gut microbiota and result in obesity, diabetes, dyslipidemia, renal complications, etc. though there are few traditional safeguards such as herbal brews to maintain the ecological stability under intestinal dysbiosis. The present article is designed to collect all the scientific facts in a place to decipher the role of the Indian traditional herbal brews used to balance gut health for centuries.

Unraveling microbial landscapes: high-throughput amplicon sequencing reveals distinct bacterial communities and potential health risks in potable springs of the Indian Himalayas.

Spring water is a vital drinking resource for residents in the Eastern Himalayas' Sikkim, India. While our initial investigations into spring water quality highlighted concerning levels of fecal coliform bacteria, the bacterial community composition (BCC) of these springs remains largely unexplored. This study sought to elucidate the BCC of Himalayan spring water, exploring its effects on water quality and delving into the unique bacterial ecology of these high-altitude springs.

A compendium of methods: Searching allele specific expression via RNA sequencing.

Diploid mammalian genome has paired alleles for each gene; typically allowing for equal expression of the two alleles within the cell/tissue. However, genetic regulatory elements and epigenetic modifications can disrupt this equality, leading to preferential expression of one allele. Examining high-confidence allele-specific expression (ASE) is vital for understanding genetic variations and their impact on major diseases like cancers and diabetes.