Exploring salivary metabolites as biomarkers in chronic craniofacial and orofacial pain: a metabolomic analysis.

Introduction: Chronic facial pain (CFP) includes a range of conditions such as musculoskeletal, neurovascular, and neuropathic disorders affecting the facial and jaw regions, often causing significant distress to patients.

Objectives: This study aims to investigate the metabolomic profile of patients with CFP, focusing on salivary metabolites as potential biomarkers for pain diagnosis and management.

Methods: Metabolomics investigation was performed using combined liquid chromatography with mass spectrometry (UPLC-MS) for metabolic profiling.

Calcitriol and Tacalcitol Modulate Th17 Differentiation Through Osteopontin Receptors: Age-Dependent Insights from a Mouse Breast Cancer Model.

Purpose: Beyond its direct anticancer effects in breast cancer (BC), vitamin D (VD) also modulates tumor progression and metastasis through immune mechanisms. T-helper 17 (Th17) cells may play a key role in these effects. This study investigates how VD influences Th17 differentiation in 4T1 and 67NR murine BC models.

Antibiotic-resistant Acinetobacter baumannii can be killed by a combination of bacteriophages and complement.

Infections caused by multidrug-resistant Acinetobacter baumannii are an emerging global health threat. Although phages have shown promising results in treating bacterial infections, the mechanisms of the combined effect of phages and innate immunity on clearing A. baumannii remain unclear.

Comparison of the microbiome of bladder urine, upper urinary tract urine, and kidney stones in patients with urolithiasis.

Introduction: It is believed that bacteria can be involved in the formation of all types of stones. The aim of study was to assess the urinary microbiome in patients with urolithiasis.

Material And Methods: The study group included 50 patients qualified for endoscopic treatment of urinary tract stones using: ureteroscopic lithotripsy (URSL), retrograde intrarenal surgery (RIRS), percutaneous nephrolithotripsy (PCNL), endoscopic combined intrarenal surgery (ECIRS).

Biological characterization and stability of three lytic wastewater-derived bacteriophages targeting multidrug-resistant Acinetobacter baumannii and A. johnsonii clinical strains.

Due to the rapidly increasing problem of pathogen resistance to drugs, including bacterial resistance to antibiotics, there is a growing need to explore new therapeutic solutions. One of the multidrug-resistant pathogens responsible for many difficult-to-treat infections is Acinetobacter baumannii. The clinical relevance of another species, A.

Hybrid nucleobase-heterocycle-2-oxindole scaffolds as innovative cell cycle modulators with potential anticancer activity.

A series of hybrid molecules 6a-6d-13a-13d combining pyrazolo[3,4-]pyrimidine or aminopurine frameworks with an oxindole moiety were designed as multitarget anticancer agents. Several compounds, especially 8b and 12a-12d, showed potent antiproliferative effects against three human cancer cell lines: A498 (kidney carcinoma), HepG-2 (hepatocellular carcinoma), and MDA-MB-231 (breast adenocarcinoma). Compounds 6b, 7b, 8b, and 12a-12c exhibited remarkable CDK6 inhibition (pIC of up to 7.

Variation in digit ratio (2D:4D) across different types of military services in Poland.

Background: The relationship between the second (2D) and fourth finger (4D) of the hand (2D:4D) is considered to be a proxy indicator of prenatal- testosterone (PT) and estrogen (PE) exposure in the first trimester of pregnancy. A lower 2D:4D indicates relatively higher PT exposure and vice versa. The 2D:4D is generally higher in women than in men.

Novel strategies for inhibiting SufA protease: the role of ester substituents and biological properties.

Aims: This study aims to develop novel antibacterial agents by targeting SufA protease, a key virulence factor in Finegoldia magna, using 1-aminoalkylphosphonate (1-AAP) diaryl esters as inhibitors.

Materials & Methods: Structural optimization of a reference inhibitor, Cbz-6-AmNpthP(OC₆H₅)₂, was performed by introducing substituents at the para position of phenyl rings: -SCH₃ (), -OCH₃ (), and -COOCH₃ (). Enzymatic assays, molecular modeling, antibacterial activity screening, and CD spectroscopy were utilized to evaluate inhibitory potency, binding interactions, functional effects, and DNA interaction.

The Apemen Faces Database (ApeFD).

The Apemen Faces Database is a novel and versatile stimulus set designed for research in behavioral biology, evolutionary psychology, and related fields. The dataset comprises 620 photorealistic, artificially generated facial images of 31 generalized hominin models, available in multiple ocular coloration variants (31 hominins x 20 color variants). Each of the 31 facial portraits is paired with geometric morphometric data and norming information that includes perceptual ratings of six constructs (Threat, Sociability, Trustworthiness, Health, Age, and Masculinity).

Expression of a human Gb3/CD77 synthase in insect and human cells: comparison of activity and glycosylation.

Glycosylation of proteins can impact their folding, stability, trafficking and enzymatic activity. Human Gb3/CD77 synthase (α1,4-galactosyltransferase, A4galt) has two occupied N-glycosylation sites. Previously, we demonstrated that the activity of recombinant enzyme relies on its N-glycosylation.

The importance of dopamine levels and single-nucleotide polymorphism within , and genes in obstructive sleep apnoea.

Background: Obstructive sleep apnoea (OSA) is a prevalent sleep disorder that contributes to serious cardiovascular comorbidities. While the mechanical aspects of OSA are well-studied, its neurobiological underpinnings remain underexplored. In this study, we investigated the role of dopamine and its genetic modulators in OSA pathophysiology.

From inert to active: Charge-compensated metallacarboranes with high toxicity and anticancer potential.

Since their initial synthesis by Hawthorne in the 1960s, inorganic metallacarboranes - most notably cobalt bis(dicarbollide), [CoSAN], have been considered chemically inert and harmless to eukaryotic cells. This reputation for inertness has supported their use in a wide range of biomedical applications, including as a boron source for boron neutron capture therapy, antifungal and Gram-positive antibacterial agents, and superchaotropic membrane carriers for impermeable biologically important molecules. In this study, we report a surprising twist: newly synthesized, iodinated and charge-compensated [CoSAN] derivatives with boron-nitrogen (-B-N-) bond show promising anticancer activity.

Pharmacogenetics of graft-versus-host disease: a path to personalized medicine.

Graft-versus-host disease (GvHD) remains a significant complication of allogeneic hematopoietic stem cell transplantation (HSCT), contributing to increased morbidity and mortality. Thus, continuous development of novel prophylactic and therapeutic approaches is crucial for GvHD prevention and management. With the current development of personalized medicine and a more patient-oriented approach, pharmacogenetics has the potential to become a critical factor in optimizing the prophylaxis and treatment of GvHD.

The examination of in vitro photosensitizing efficacy of aloe-emodin loaded liposome following photodynamic therapy on melanoma cell lines.

This study focused on investigating the enhanced photoactive properties of liposomal aloe-emodin for photodynamic treatment (PDT). Aloe-emodin (A-E) was encapsulated in liposomes by using the copper ion gradient method. This study aimed to highlight the function of naturally occurring photoactive plant-based derivatives in liposomal formulations as simple alternative photosensitizers (PS) for melanoma photodynamic therapy (PDT).

Vitamin D and SIRT1 activator SRT2104 cooperate in antiproliferative activity in lung cancer through ferroptosis induction.

A number of studies showed that vitamin D reveals antiproliferative activity against cancer cells, however some lung cancer cells are more sensitive than others. Secondly, there are some controversies around the role of SIRT1 in lung cancer: on one hand it was demonstrated that SIRT1 contributed to the development of lung cancer, on the other hand SIRT1 was showed to be downregulated in lung cancer. Third, it was demonstrated that VDR is posttranslationally modified via deacetylation by SIRT1 what potentiated VDR activity.

Phage- and enzybiotic-coated urinary catheters to prevent recurrent multidrug-resistant bacterial infections.

Introduction: Catheter-associated urinary tract infections (CAUTIs) are among the most prevalent hospital-acquired infections, posing a serious clinical and economic burden, particularly in the context of rising antibiotic resistance. Biofilm formation on indwelling catheters by multidrug-resistant uropathogens further complicates treatment and prevention, underscoring the urgent need for alternative, non-antibiotic solutions.

Areas Covered: This article explores the potential of bacteriophage- and enzybiotic-coated urinary catheters as an innovative strategy to prevent recurrent bacterial infections.

The Potential of Normobaric Oxygen Therapy to Enhance Erythropoiesis, Reduce Oxidative Stress, and Modulate Immune Function in Colorectal Cancer Patients Undergoing Chemotherapy: Study Protocol for a Prospective, Randomized, Double-Blind, Placebo-Controlled Trial (NBO-ONCO).

Colorectal cancer (CRC) patients undergoing chemotherapy often experience anemia, oxidative stress, and immune suppression, significantly impacting their quality of life and treatment outcomes. Normobaric oxygen (NBO) therapy, which delivers oxygen at atmospheric pressure with an elevated oxygen concentration, has shown the potential to enhance erythropoiesis, reduce oxidative stress, and modulate immune function. However, its efficacy in CRC patients remains underexplored.

Characteristics of Isolated from Humans and Animals.

(. ) is an opportunistic coagulase-negative staphylococcus (CoNS) known to cause urinary tract infections in humans and is increasingly recognized in veterinary medicine. The aim of this study was to provide an epidemiological characterization of .

Venetoclax-Based Regimens in CLL: Immunoglobulin G Levels, Absolute Neutrophil Counts, and Infectious Complications.

Chronic lymphocytic leukemia (CLL) is a prevalent hematologic malignancy that predominantly affects elderly individuals, posing significant clinical challenges due to patient comorbidities and inherent resistance to conventional chemotherapy. The emergence of targeted therapies combining venetoclax, a selective inhibitor of the anti-apoptotic protein BCL-2, with anti-CD20 monoclonal antibodies has dramatically transformed the treatment landscape. This retrospective observational study analyzed the differential impacts of first-line venetoclax-obinutuzumab (VenO) and second-line venetoclax-rituximab (VenR) on immunoglobulin G (IgG) levels and absolute neutrophil count (ANC) in CLL patients.

Proteolytic Activity Inhibition in Gingival Fluid by Cysteine Protease Inhibitors Obtained from Egg White and Extract: An In Vitro Study.

: Gingipains produced by have been shown to be directly related to periodontal tissue degradation and are significant molecular targets in therapy of periodontitis. Blocking the activity of these enzymes should reduce survival of this pathogen and mitigate the effects of inflammation in periodontitis. Therefore, gingipains inhibitors and specific antibodies could be recommended in the treatment of periodontitis.

Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Bacterial Viruses Subcommittee, 2025.

This article summarises the activities of the International Committee on Taxonomy of Viruses Bacterial Viruses Subcommittee, detailing developments in the classification of bacterial viruses. We provide here an overview of all new, abolished, moved and renamed taxa proposed in 2024, approved by the Executive Committee, and ratified by membership vote in 2025. Through the collective efforts of 74 international contributors of taxonomy proposals in this round, 43 ratified proposals have led to the creation of one new phylum, one class, four orders, 33 families, 14 subfamilies, 194 genera and 995 species.

Reconstruction of outer glycolipid synthesis pathways from Porphyromonas gingivalis in Escherichia coli for production of a vaccine candidate.

Background: Porphyromonas gingivalis is a major human oral opportunistic pathogen and a key etiological agent of periodontal disease, contributing to inflammation and bone loss in the oral cavity. Periodontitis is not limited to oral health complications; it has also been associated with a range of systemic conditions, including coronary heart disease (CAD), respiratory disease, rheumatoid arthritis, chronic kidney disease (CKD), and certain types of cancer.

Objectives: Immunization-based prevention of periodontitis appears to be a promising strategy; however, no vaccine is currently available for commercial use.

A systematic review of miRNA expression in schizophrenia spectrum disorders across the blood and the brain.

Several studies have investigated whether individuals with schizophrenia spectrum disorders (SSD) show altered microRNA expression. However, findings in this field have not been synthesized so far. Therefore, we performed a systematic review of studies measuring microRNA levels in the peripheral blood and brain samples of individuals with SSD and healthy controls.