Advances in the direct conversion of CH and CO into acetic acid over bimetallic catalysts supported on H-ZSM-5.

This study developed heterogeneous catalysts composed of ZnO and CeO supported on H-ZSM-5 for the direct conversion of methane (CH) and carbon dioxide (CO) into acetic acid. The acid-base and electronic properties were modulated through oxide impregnation and reduction, aiming to create active sites capable of simultaneously activating both reactants. The samples were characterized by XRD, N physisorption, HRTEM/EDS, NH-TPD, CO-TPD, TPR, FTIR, XPS, CO-DRIFTS, and TGA, and tested in a batch reactor at 300 °C and 10 bar.

Zinc oxide-curcumin nanoparticles supplementation during oocyte maturation improves bovine embryo production.

This work investigated the effect of zinc oxide nanoparticles functionalized with curcumin (ZnO+CUR) supplementation during the maturation (IVM) of bovine oocytes on the embryo production and the cellular antioxidant response. A total of 1,625 cumulus-oocyte complexes (COCs) were cultured in the maturation medium in the absence (0 µM - control) or presence of different concentrations of ZnO+CUR (3 µM, 6 µM or 12 µM). After IVM, COCs were destined either to 1) embryo production or 2) analysis of reactive oxygen species production, superoxide dismutase (SOD) activity, catalase (CAT) activity and total antioxidant capacity (FRAP).

Factors Associated With the Continuation of Breastfeeding Beyond 12 Months of Age in Brazilian Families.

Aim: To measure the prevalence of BF amongst Brazilian children aged 12-24 months, assess associated social determinants and evaluate the impact of maternal knowledge about its benefits.

Methods: A cross-sectional study was conducted between September and December 2024, using an online questionnaire completed by Brazilian mothers with children aged 12-24 months. Sociodemographic data were collected, including maternal and child age, education level, marital status, ethnicity, household income and employment status.

Prospective validation of podoplanin expression as a diagnostic biomarker of acute promyelocytic leukemia.

Acute promyelocytic leukemia (APL) is a medical emergency that needs immediate diagnosis and treatment. Podoplanin, a transmembrane glycoprotein that binds CLEC-2 on platelets, was recently demonstrated to be abnormally expressed in leukemic blasts in APL, as opposed to other forms of AML, in a study using thawed primary cells. This study aimed to explore and validate the diagnostic accuracy of measuring podoplanin expression by flow cytometry in the differential diagnosis of APL and other forms of acute myeloid leukemia (AML) as part of the diagnostic work-up of all cases suspected of AML in an academic hematology center.

Developing a Latin American Delphi consensus on vaccination for respiratory diseases in older adults.

Background: Respiratory infections significantly impact older adults in Latin America, highlighting the need for regionally adapted consensus-based vaccination recommendations to guide preventive strategies. This study aimed to develop a consensus among Latin American experts on vaccination against respiratory diseases in older adults in the region, including influenza, Streptococcus pneumoniae pneumonia, COVID-19, respiratory syncytial virus (RSV), and pertussis.

Methods: A two-round Delphi methodology was employed, involving 35 specialists from various medical fields.

Cooperative Enhancement of Aldoxime Dehydratase Stability through Whole-Cell Immobilization and Flow Reactor Integration.

This study investigates the synthesis of aromatic nitriles using an evolved variant of OxdF1 (L318F/F306Y), an aldoxime dehydratase from Pseudomonas putida F1, engineered for improved catalytic efficiency toward benzaldehyde oxime. The double OxdF1 (L318F/F306Y) mutant effectively catalyzes the conversion of various benzaldoxime derivatives to the corresponding nitriles. Due to the enzyme's inherent instability, immobilized whole-cell systems are employed in a flow reactor to improve its stability and broaden its applicability, with the biotransformation of benzaldehyde oxime and 2,6-difluorobenzaldehyde oxime serving as case studies.

Photobiomodulation and platelet-rich fibrin in the gastrocnemius muscle submitted to calcaneal tendinopathy in rats.

The study investigated the impact of different treatments on recovery from calcaneal tendinopathy in rats, focusing on the gastrocnemius muscle. Tendinopathy is caused by repetitive overload, leading to structural collagen damage and chronic muscle inflammation. Three therapeutic approaches were compared: photobiomodulation (PBM), advanced platelet-rich fibrin (A-PRF) injection, and a combination of the two.

Violacein-Loaded Outer Membrane Vesicles from Exhibit Potent Anti-Melanoma Activity and .

Violacein exhibits antitumor activity, indicating potential for future clinical application. However, an efficient delivery system is required for the clinical use of this hydrophobic compound. Effective delivery systems can enhance the solubility and bioavailability of hydrophobic compounds like violacein, facilitating its clinical application for antitumor therapy.

Impacts on Gene Expression Profiles Associated with the Most Widely Used Pesticides in Central Brazil.

While agriculture is essential for food security, the intensive use of pesticides in modern farming practices raises concerns on their impact, in particular from a One Health perspective. In 2024, Brazil approved 663 new pesticides, a 19% increase in comparison with 2023. The occupational exposure of rural workers is known to be associated with a range of health outcomes, including cancer.

Reference Values for the Dynamic Evaluation Method of Lower Limb Joint Alignment (MADAAMI II) for Dancers.

The aim of this study was to propose reference values for the Dynamic Evaluation Method of Lower Limb Joint Alignment (MADAAMI II). During the 2023 Joinville Dance Festival, 346 dancers (315 females and 31 males), aged between 15 and 58 years, with a minimum of 5 years of ballet experience, were randomly evaluated. The dancers were filmed performing the three-step sequence (demi plié, grand plié, and fondu) of the MADAAMI II, in the first foot position, with self-selected turnout and at 120 degrees.

Solanum glycoalkaloid solamargine inhibits growth of pediatric high-grade glioma cells under normoxia and hypoxia conditions.

Pediatric high-grade gliomas remain a significant therapeutic challenge due to their resistance to conventional treatments. The aim of this study was to investigate the cytotoxic potential of solamargine (SM), a natural glycoalkaloid, alone and in combination with the chemotherapeutic agent temozolomide (TMZ) against the human KNS-42 glioma cell line. Solamargine significantly reduced cell viability and proliferation in a concentration-, time-, and hypoxia-dependent manner, while selectively sparing non-tumor human astrocytes (NHA).

Progressive Vision Loss From Uveal Melanoma with Extraocular Extension: A Case Report.

BACKGROUND Uveal melanoma is the most common primary intraocular malignancy in adults, often diagnosed late in resource-limited settings. The diagnosis is made through a combination of clinical ophthalmologic examination, B-mode ultrasound, and histopathological study. This report details a case of a 67-year-old woman with progressive vision loss and ocular pain due to an inferomedial uveal melanoma to highlight therapeutic limitations from delayed diagnosis.

New alternatives in photoprotection: Preparation and evaluation of lamellar silicate derivatives and their use as sunscreens.

Objective: The objective of this work is to investigate different sunscreens and Viscogel group organoclays for the preparation of new intercalated sunscreens to improve the effectiveness and safety in photoprotection using new approach methodology (NAMs).

Methods: For this study, we examined Diethylamino hydroxybenzoyl hexyl benzoate (DHHB), octyl methoxycinnamate (OMC), Bemotrizinol (BEMT) and Viscogel S4®, S7®, and B8® using a set of Saccharomyces cerevisiae mutant strains that are sensitive to UVA, UVB and Solar Simulated Light (SSL) to evaluate their photoprotective and mutagenic potential. Additionally, we developed delaminated nanocomposites by chemical intercalation reactions followed by ultrasonic treatment to enhance clay exfoliation.

Excessive Daytime Sleepiness and Its Associated Factors Among Male Road Transport Workers in Brazil.

This study aimed to estimate the occurrence of excessive daytime sleepiness (EDS) and its associated factors among male road transport workers. A cross-sectional study was conducted with a non-probabilistic sample of 414 drivers recruited at gas stations and parking lots in Formosa and Rio Verde, Goiás, Brazil, in 2024. The presence of EDS was evaluated using the Epworth Sleepiness Scale, and the investigated associated factors included demographic, socioeconomic, behavioural, health and professional characteristics.

Enhanced Energized Dispersive-Guided Extraction Using Doehlert Matrix Optimization and Antioxidant Evaluation of Phenolic Compounds From Herbal Leaves by Using High-Performance Liquid Chromatography.

Secondary metabolites are important bioactive compounds for diet and medicine. This study optimizes the extraction of hydroethanolic herbal extracts using an EDGE (Energized Dispersive Guided Extraction) system, evaluates their antioxidant capacity, and analyzes correlations among antioxidant activity, total phenolic content, and individual compounds. A Doehlert matrix design was used to optimize extraction, having temperature and time as independent variables, and total phenolic content (mg GAE/g) as the response, quantified via the Folin-Ciocalteu method.

Prevalence of biofilm in chronic wounds: systematic review with meta-analysis.

Background: To estimate the prevalence of biofilms in chronic wounds.

Methods: The authors performed a systematic review of prevalence studies and meta-analysis, structured according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses guidelines. Articles were searched in Scopus (Elsevier), Web of Science (Clarivate), MEDLINE/PubMed (National Institutes of Health), and Embase (Elsevier) databases.

The trial design of the concurrent optical and magnetic stimulation (COMS) therapy study for refractory diabetic foot ulcers (MAVERICKS): a multicenter, randomized, sham-controlled, double-blind investigational device exemption clinical study.

Background: Diabetic foot ulcers (DFUs) are a major clinical challenge, particularly among patients with refractory ulcers, that often lead to severe complications such as infection, amputation, and high mortality. Innovations supported by strong clinical evidence have the potential to improve healing outcomes, enhance quality of life, and reduce the economic burden on individuals and health care systems.

Objective: To describe the design of the concurrent optical and magnetic stimulation (COMS) therapy Investigational Device Exemption (IDE) study for refractory DFUs (MAVERICKS) trial.

Statins as Antifungal Agents: A Review on Drug Repurposing and Nanotechnology-Driven Delivery Strategies.

This review highlights the integration of drug repurposing and nanotechnology-driven delivery strategies as innovative approaches to enhance the antifungal activity of statins against mucosal candidiasis, providing a framework for future translational research and clinical application. The rising prevalence of antifungal resistance and virulence factors of Candida albicans underscore the limitations of current therapies. Statins, commonly used as lipid-lowering agents, have emerged as attractive repurposed drug candidates due to their ability to interfere with fungal ergosterol biosynthesis and Ras-mediated signaling pathways.

A Sensitive and Comprehensive LC-MS/MS Method for the Analysis of Hallucinogens, Synthetic Cathinones, and Synthetic Cannabinoids in Urine Samples.

The laboratory analysis of new psychoactive substances and related drugs is crucial for accurate clinical and forensic diagnosis of poisonings. Given this, a new LC-MS/MS method for analyzing hallucinogens, synthetic cathinones, and synthetic cannabinoids in urine was developed. Urine samples were extracted using a liquid-liquid extraction protocol optimized via a multivariate experimental design.

Decoding Tupiperla illiesi Froehlich 1998 (Plecoptera: Gripopterygidae): Insights into Morphological Variation and Molecular Species Delimitation.

This study addresses historical uncertainties regarding morphological variation in the paraprocts of Tupiperla illiesi, a stonefly with a complex taxonomic history. We tested whether these variations represent phenotypic plasticity or distinct species using integrative taxonomy. Adult gripopterygids were collected from Estação Biológica de Boracéia utilizing Malaise and light traps.

Inflammatory gene expression profile of oral plasmablastic lymphoma.

Plasmablastic lymphoma (PBL) is a rare and aggressive non-Hodgkin lymphoma with a poor prognosis and short survival rates. It is classified as a large B-cell lymphoma subtype, but carries a plasmacytic immunophenotype. Therefore, PBL has pathogenetic overlaps with diffuse large B-cell lymphoma not otherwise specified (DLBCL NOS) and plasma cell neoplasms (PCNs).