Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
We have developed a method by which nuclear shells containing nucleoli can be isolated from membrane-depleted rat liver nuclei. This method involves the removal of the internal chromatin. This chromatin is expelled from the nuclear shell using combinations of low and high ionic strength buffers. The expelled internal part is subsequently digested with DNase I or micrococcal nuclease. Examination by electron microscopy of the nuclear and the nucleolar structures at various steps of the isolation procedure shows that the nucleoli are anchored in the peripheral lamina by a pedicle that is continuous with an intranucleolar network. This network is masked in situ by nucleolar granules. The pedicle and the network which support the nucleolar DNA are composed mainly of non-histone proteins insoluble in 2M NaCl.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1768-322x.1985.tb00326.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Safety of artificial intelligence-assisted optical diagnosis for leaving colorectal polyps in situ during colonoscopy (PRACTICE): a non-inferiority, randomised controlled trial.
Lancet Gastroenterol Hepatol
October 2025
Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Italy; Endoscopy Unit, IRCCS Humanitas Research Hospital, Rozzano, Italy.
Background: Guidelines recommend leaving in situ rectosigmoid polyps diagnosed during colonoscopy that are 5 mm or smaller if the endoscopist optically predicts them to be non-neoplastic. However, no randomised controlled trial has been done to examine the efficacy and safety of this strategy.
Methods: This open-label, multicentre, non-inferiority, randomised controlled trial enrolled adults age 18 years or older undergoing colonoscopy for screening, surveillance, or clinical indications across four Italian centres.
Case report: Duplicate renal ureteral malformation with renal tumor, ureteral tumor, renal rupture, calculi and infection.
Int J Surg Case Rep
September 2025
Department of Urology, Shandong Provincial Third Hospital, Shandong University, Jinan, 250012, China; Shandong Stone Disease Prevention and Treatment Center, Jinan, 250012, China. Electronic address:
Introduction: The Multiple primary malignant tumors (MPMT) refers to the occurrence of two primary malignant tumors in the same organ or organs in the same patient at the same time. However, MPMT is rare in the urinary system. Congenital urinary tract anomalies (e.
View Article and Find Full Text PDFHyperelastic characterization deep indentation.
Soft Matter
September 2025
Mechanical Engineering Department, Institute of Applied Mathematics School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Hyperelastic material characterization is crucial for sensing and understanding the behavior of soft materials-such as tissues, rubbers, hydrogels, and polymers-under quasi-static loading before failure. Traditional methods typically rely on uniaxial tensile tests, which require the cumbersome preparation of dumbbell-shaped samples for clamping in a uniaxial testing machine. In contrast, indentation-based methods, which are non-destructive and can be conducted without sample preparation, remain underexplored.
View Article and Find Full Text PDFMetal-Free Organocatalytic Formylation by CO-Masked Carbene Functionalized Graphene Oxide Nanosheets.
ChemSusChem
August 2025
Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montréal, QC, H3A 2K6, Canada.
Despite considerable scientific advancements, there is an urgent need for sustainable, cost-effective, and efficient methods for chemically transforming CO into valuable chemicals. A stable heterogeneous platform is presented that incorporates four key innovations: 1) the first Tröger's base (TB) chemistry in solids via selective four-electron reductive functionalization of CO, 2) an effective heterogeneous organocatalyst for the chemoselective formylation of both NH and SH functionalities with CO, 3) a methodology for metal-free heterogeneous S-formylation of bioactive thiols, and 4) a direct covalent immobilization of CO-protected N-heterocyclic carbenes (NHCs) on graphene oxide nanosheets (GONs). The CO-protected catalyst is developed by covalently attaching imidazole (Im) to GONs and functionalizing them with dimethyl carbonate.
View Article and Find Full Text PDFBioorthogonal reaction mediated size transformation clustered nanosystems for potentiating bioimaging and drug delivery.
J Mater Chem B
August 2025
Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, School of Life and Health Sciences, Hubei University of Technology, Wuhan 430068, China.
Continuous advances have been witnessed in the booming of size variable nanosystems for molecular imaging and therapy. These nanosystems usually exhibit size transformation, which promotes optimized biodistribution, retention and penetration in lesions. Bioorthogonal reactions have been introduced as a useful tool to develop size variable nanosystems.
View Article and Find Full Text PDF