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Proc Natl Acad Sci U S A
September 2025
Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
Agonist-induced interaction of G protein-coupled receptors (GPCRs) with β-arrestins (βarrs) is a critical mechanism that regulates the spatiotemporal pattern of receptor localization and signaling. While the underlying mechanism governing GPCR-βarr interaction is primarily conserved and involves receptor activation and phosphorylation, there are several examples of receptor-specific fine-tuning of βarr-mediated functional outcomes. Considering the key contribution of conformational plasticity of βarrs in driving receptor-specific functional responses, it is important to develop novel sensors capable of reporting distinct βarr conformations in cellular context.
View Article and Find Full Text PDFCell Biochem Biophys
September 2025
Medical Biotechnology Research Center, School of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran.
In cardiovascular research, melatonin has shown promise in exhibiting antifibrotic properties and modulating endoplasmic reticulum (ER) stress. However, the exact mechanism by which it influences myocardial fibrosis has not been fully clarified. Therefore, this research aimed to investigate the inhibitory effect of melatonin on the progression of myocardial fibrosis through a mechanism involving the BIP/PERK/CHOP signaling pathway, both in silico and in vivo experimental models.
View Article and Find Full Text PDFAppl Biochem Biotechnol
September 2025
AVT - Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, Aachen, 52074, Germany.
Microbial co-cultures provide significant advantages over commonly used axenic cultures in biotechnological processes, including increased productivity and access to novel natural products. However, differentiated quantification of the microorganisms in co-cultures remains challenging using conventional measurement techniques. To address this, a fluorescence-based approach was developed to enable the differentiated online monitoring of microbial growth in co-cultures.
View Article and Find Full Text PDFLasers Med Sci
September 2025
Department of Otolaryngology Head and Neck Surgery, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, 71 Hexi Street, Nanjing 210019, Jiangsu, China.
To evaluated the efficacy of photodynamic therapy (PDT) in improving laryngeal mucosal wound scar healing in vivo and investigated its underlying mechanisms. Laryngeal mucosal wounds were induced in Sprague-Dawley rats. Two weeks post-injury, PDT was administered via intraperitoneal injection of 100 mg/kg 5-aminolevulinic acid (5-ALA) and 635-nm red laser irradiation at varying energy doses (15, 30, and 45 J/cm²).
View Article and Find Full Text PDFCurr Microbiol
September 2025
Department of Health Sciences, Università del Piemonte Orientale UPO, Corso Trieste 15/A, 28100, Novara, Italy.
A Python-scripted software tool has been developed to help study the heterogeneity of gene changes, markedly or moderately expressed, when several experimental conditions are compared. The analysis workflow encloses a scorecard that groups genes based on relative fold-change and statistical significance, providing additional functions that facilitate knowledge extraction. The scorecard reports highlight unique patterns of gene regulation, such as genes whose expression is consistently up- or down-regulated across experiments, all of which are supported by graphs and summaries to characterize the dataset under investigation.
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