Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Artificial cytoskeletons are constructed to study the structure and function of eukaryotic cells. Metal-organic frameworks (MOFs) provide a strong foundation for the construction of artificial cytoskeleton by encapsulating enzyme, yet challenges such as random enzyme distribution and poor catalytic efficiency, impede the development of artificial cytoskeleton technologies. Herein, a multilayer MOFs-based programmable artificial cytoskeleton was constructed through a heterogeneous interfacial growth method, utilizing hierarchical encapsulation of enzymes to facilitate tandem biocatalytic reactions. Specific cascade channels were constructed by adjusting the size and pore length of multilayer MOFs based artificial cytoskeleton, which improves the interfacial contacts and enhances mass transfer efficiency, achieving precise regulation of enzyme activity for mimicking cellular metabolism. Harnessing these advantages, an artificial cytoskeleton-based immunosensor was developed to on-site detect isocarbophos pesticide, which achieves 51-fold enhancement in sensitivity and precise detection of target without bulky instrumentation. These advances highlight the advantages of artificial cytoskeleton in efficient catalysis and mimicking of biological functions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2025.117948 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multilayer metal-organic frameworks-based artificial cytoskeleton for boosting immunosensors performance.
Biosens Bioelectron
September 2025
State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, PR China. Electronic address:
Artificial cytoskeletons are constructed to study the structure and function of eukaryotic cells. Metal-organic frameworks (MOFs) provide a strong foundation for the construction of artificial cytoskeleton by encapsulating enzyme, yet challenges such as random enzyme distribution and poor catalytic efficiency, impede the development of artificial cytoskeleton technologies. Herein, a multilayer MOFs-based programmable artificial cytoskeleton was constructed through a heterogeneous interfacial growth method, utilizing hierarchical encapsulation of enzymes to facilitate tandem biocatalytic reactions.
View Article and Find Full Text PDFStu2 performs an essential kinetochore function independent of its microtubule polymerase activity.
J Cell Biol
October 2025
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA.
ch-TOG family proteins, including the budding yeast Stu2, are essential for spindle formation and chromosome segregation. Such functions depend on an array of activities ranging from microtubule nucleation, polymerization, and depolymerization to conferring tension sensitivity to kinetochores. This functional diversity makes it challenging to dissect these various functions and understand their relative importance.
View Article and Find Full Text PDFMechanical interactions impact the functions of immune cells and their application in immunoengineering.
Adv Ther (Weinh)
June 2025
Department of Materials Science and Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, USA.
Immune cells experience a wide range of modes and magnitudes of mechanical forces as they infiltrate tissues and physically interact with other cells. Biophysical forces influence cell phenotypes through mechanosensing of the cytoskeleton, cell adhesion, catch and slip bonds, and mechanically gated ion channels. As a result, different mechanical environments impact the function and expression of immune cell receptors, which subsequently affects local and systemic immune responses.
View Article and Find Full Text PDFHippocampal tau distribution in primary age-related tauopathy: The Vantaa 85+ Study.
Alzheimers Dement
August 2025
Department of Pathology, University of Helsinki, Helsinki, Finland.
Introduction: In contrast to Alzheimer's disease (AD), in which initial neurofibrillary tangles (NFT) are mainly limited to the (trans)entorhinal region (EC) and CA1/prosubiculum, primary age-related tauopathy (PART) has been suggested to exhibit an early predisposition to NFTs in the hippocampal CA2 subregion.
Methods: We created an artificial intelligence model that recognizes and quantifies NFTs of three different maturity levels in different hippocampal subfields. This model was applied to a population-based Vantaa 85+ cohort, including hippocampal tau-immunostained sections from 210 individuals aged ≥ 85 years.
Evidence for Motility Determinants in the Kinesin-1 Minimal Motor Core Domain From Tether Variations.
Cytoskeleton (Hoboken)
August 2025
Department of Life Sciences Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
Kinesin-1 is a dimeric motor protein that moves towards the microtubule plus-end in a hand-over-hand fashion. However, the minimal motor domain of kinesin-1 is a single head, and the mechanism by which minimal motor domains generate the force for directional movement remains poorly understood. Here, we engineered artificial tethers (polyethylene glycol, single-stranded DNA, or double-stranded DNA) within the motor domain to investigate whether tether properties such as charge, length, and stiffness affect the motility of teams of kinesin-1 monomers.
View Article and Find Full Text PDF