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http://dx.doi.org/10.1111/1756-185x.70397 | DOI Listing |
J Autoimmun
September 2025
Garvan Institute of Medical Research, Darlinghurst, NSW, Australia; Cellular Genomics Futures Institute & School of Biomedical Sciences, UNSW Sydney, Australia. Electronic address:
Background: In autoimmune disease it is not understood how self-reactive B cells escape immune tolerance checkpoints to produce pathogenic autoantibodies.
Objective: In patients with demyelinating polyneuropathy caused by IgM autoantibodies against myelin associated glycoprotein (MAG) and the sulphated trisaccharide CD57, we aimed to test the hypothesis that B cells making the autoantibody escaped tolerance by acquiring lymphoma driver somatic mutations.
Methods: Deep single-cell RNA, DNA, flow cytometric and antibody specificity analysis of blood from three patients with MAG neuropathy.
STAR Protoc
September 2025
Macrophage Lab, Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, Seoul 03080, Republic of Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea; Cancer Researc
Interstitial macrophages increase significantly during lung metastasis and may contribute to tumor dissemination. However, isolating them is challenging due to their localization within lung tissue and phenotypic overlap with other immune cells. Here, we present a protocol for isolating and characterizing murine interstitial macrophages.
View Article and Find Full Text PDFACS Appl Bio Mater
September 2025
Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
The development of multifunctional nanoplatforms capable of drug delivery and real-time cellular imaging remains a key challenge in cancer theranostics. Herein, we report the development of a casein-protected maleic acid-derived nitrogen-doped carbon dot-based luminescent nanoplatform (MNCD@Cas NPs) for efficient delivery of the anticancer drug doxorubicin hydrochloride (DOX) to triple-negative breast cancer cells. Synthesized via a facile two-step method, the MNCD@Cas NPs exhibit bright blue fluorescence (λ = 390 nm), high water dispersibility, excellent colloidal stability, and substantial DOX loading capacity (∼84%) driven by electrostatic interactions.
View Article and Find Full Text PDFInt J Rheum Dis
September 2025
Allergy Immunology Unit, Department of Pediatrics, Advanced Pediatrics Centre, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Hum Immunol
September 2025
Department of Pathology and Laboratory Medicine, University of Louisville, Louisville, KY, USA; Medical Affairs, Werfen, Wakesha, WI, USA.
Single Antigen Bead (SAB) assays are the cornerstone of HLA antibody detection in transplant immunology. However, discrepancies in mean/median fluorescence intensity (MFI) values between the two commercially available assays raise concerns about result interpretation and assay interoperability. Using 281 well-characterized serum samples positive for a broad range of HLA antibodies, we evaluated 118 core HLA antibody specificities for MFI concordance across platforms.
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