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The purpose of this study was to reveal the effect and mechanism of L-Glu treatment on the enrichment of phenolic compounds in wheat sprouts under NaCl stress. Results of data independent acquisition (DIA) proteomics analysis showed that there were 978 DAPs in NaCl compared to CK, and 3813, 202, and 3413 DAPs in L-Glu, glutamate receptor antagonist 6,7-dinitriquinoxaline-2,3-dinoe (DNQX), and L-Glu plus DNQX compared to NaCl, respectively. These DAPs were mainly related to cellular metabolism, organic matter metabolism, and polyphenol compound metabolism. Based on KEGG analysis, DAPs were mainly enriched in metabolic pathways such as photosynthesis and phenylpropanoid biosynthesis. In addition, upregulation of phenylalanine ammonia lyase and 4-coumaric acid coenzyme A ligase proteins promoted the accumulation of phenolic compounds in wheat sprouts. This study revealed the mechanism of L-Glu-induced phenolics enrichment in wheat sprouts under NaCl stress, providing a theoretical basis for the growth of wheat under adversity.
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http://dx.doi.org/10.1016/j.foodchem.2025.145972 | DOI Listing |
Food Chem
August 2025
College of Food Science and Technology, Henan Agricultural University/Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, Zhengzhou 450002, China. Electronic address:
The purpose of this study was to reveal the effect and mechanism of L-Glu treatment on the enrichment of phenolic compounds in wheat sprouts under NaCl stress. Results of data independent acquisition (DIA) proteomics analysis showed that there were 978 DAPs in NaCl compared to CK, and 3813, 202, and 3413 DAPs in L-Glu, glutamate receptor antagonist 6,7-dinitriquinoxaline-2,3-dinoe (DNQX), and L-Glu plus DNQX compared to NaCl, respectively. These DAPs were mainly related to cellular metabolism, organic matter metabolism, and polyphenol compound metabolism.
View Article and Find Full Text PDFFront Plant Sci
August 2025
Plant Biotechnology Center, College of Agronomy, Jilin Agriculture University, Changchun, Jilin, China.
Salinized soil can significantly hinder soybean growth, leading to a reduction in overall yield. To address this issue, identifying key genes related to salt tolerance in soybeans is essential for improving their resistance to salinity and ensuring sustainable development of soybean production. While current research predominantly focuses on salt tolerance during the seedling stage, there is still a lack of comprehensive studies on the genes involved in salt tolerance during the germination stage.
View Article and Find Full Text PDFFront Plant Sci
July 2025
Academy of Animal Husbandry and Veterinary Science, Qinghai University, Xining, China.
Introduction: This study aimed to investigate the seed germination characteristics and metabolic response mechanisms of under saline-alkali stress, with the goal of elucidating its physiological and molecular mechanisms of salt-alkali tolerance.
Methods: It was systematically analyzed the germination characteristics of four germplasm resources (YSZ, XHZ, Shoulu, and Longzhong 1) from different origins with various concentrations of individual salts (NaCl, NaSO, NaHCO) and compound saline-alkali stress treatments. Additionally, the metabolite changes in the domesticated species under saline-alkali stress were examined using metabolomics technology.
Foods
June 2025
Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy.
This study investigated the phytochemical content of melon sprouts obtained from by-product seeds of fruit processing and the elicitation effect obtained by the application of salinity to the growing substrate. Seeds from two melon Cultivars (Thales and SV9424ML) were sprouted at 0, 12.5, 25, and 50 mM NaCl concentrations (Salt).
View Article and Find Full Text PDFPlants (Basel)
March 2025
Institute of Biology and Agricultural Biotechnology (IBBA), National Research Council, Pisa Unit, 56124 Pisa, Italy.
Willd. is a halophytic plant valued for its nutritional and nutraceutical properties, as well as its adaptability to diverse soil and climatic conditions. Biostimulant application enhances plant quality and resilience under adverse environmental conditions.
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