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Article Abstract

The purpose of this study was to reveal the effect and mechanism of L-Glu treatment on the enrichment of phenolic compounds in wheat sprouts under NaCl stress. Results of data independent acquisition (DIA) proteomics analysis showed that there were 978 DAPs in NaCl compared to CK, and 3813, 202, and 3413 DAPs in L-Glu, glutamate receptor antagonist 6,7-dinitriquinoxaline-2,3-dinoe (DNQX), and L-Glu plus DNQX compared to NaCl, respectively. These DAPs were mainly related to cellular metabolism, organic matter metabolism, and polyphenol compound metabolism. Based on KEGG analysis, DAPs were mainly enriched in metabolic pathways such as photosynthesis and phenylpropanoid biosynthesis. In addition, upregulation of phenylalanine ammonia lyase and 4-coumaric acid coenzyme A ligase proteins promoted the accumulation of phenolic compounds in wheat sprouts. This study revealed the mechanism of L-Glu-induced phenolics enrichment in wheat sprouts under NaCl stress, providing a theoretical basis for the growth of wheat under adversity.

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http://dx.doi.org/10.1016/j.foodchem.2025.145972DOI Listing

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