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In this work, a natural zwitterionic, betaine with varied dosages (4%, 6%, 8%, 10%, and 12%, w/v) was used to improve the freeze-thaw stability of frozen surimi gels. Specifically, Group 8B-S (8% betaine, w/v) increased gel strength and storage modulus of frozen surimi gels by 25.03% and 26.86% compared with the control group, respectively. Besides, the findings indicated that betaine also showed a versatile cryoprotection effect on frozen soy protein gels and agar gels, indicated by the relatively high gel strength. Differential scanning calorimetry (DSC) measurements showed that 8% betaine significantly reduced freezing point and freezable water content by 52.03% and 14.94% compared with the control group, respectively. Furthermore, the results of Raman spectroscopy and molecular dynamics simulations demonstrated that the ionic solvation effects of betaine disrupted the network, number, and lifetime of hydrogen bonds within water molecules, thereby improving ice recrystallization inhibition activity. This work establishes a theoretical framework for the creation of phosphorus-free antifreeze agents used in high-quality frozen food gels.
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http://dx.doi.org/10.1111/1750-3841.70534 | DOI Listing |
J Food Sci
September 2025
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, China.
In this work, a natural zwitterionic, betaine with varied dosages (4%, 6%, 8%, 10%, and 12%, w/v) was used to improve the freeze-thaw stability of frozen surimi gels. Specifically, Group 8B-S (8% betaine, w/v) increased gel strength and storage modulus of frozen surimi gels by 25.03% and 26.
View Article and Find Full Text PDFNat Commun
August 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Non-canonical amino acids (ncAAs) bearing diverse functional groups hold transformative potential in drug discovery, protein engineering, and biomaterial science. However, their industrial-scale production remains constrained by the inefficiency, high cost, and environmental burden of conventional chemical and enzymatic methods. Here, we present a modular multi-enzyme cascade platform that leverages glycerol-an abundant and sustainable byproduct of biodiesel production-as a low-cost substrate for ncAAs synthesis.
View Article and Find Full Text PDFis a non-parasitic roundworm widely utilized as a versatile model organism for studying fundamental biological processes. Despite the availability of multiple cryopreservation methods, variations in the selection of developmental stage, cryoprotectant composition, and storage conditions may sometimes cause inconsistencies and uncertainty among researchers. In this study, we followed a step-by-step approach to optimize the cryopreservation process.
View Article and Find Full Text PDFFEMS Yeast Res
January 2025
Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine.
Glycerol, a by-product of biodiesel production, is a versatile polyol used in various industries. Yeasts play a crucial role in converting glycerol into biofuels and value-added products, offering sustainable alternatives to chemical synthesis. This review explores glycerol metabolism in yeasts, focusing on its bioconversion into ethanol, isopropanol, lipids, organic acids, and polyols.
View Article and Find Full Text PDFACS Synth Biol
August 2025
Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, United States.
Cell-free gene expression systems derived from bacterial lysates enable the expression of biosynthetic pathways from inexpensive and easily prepared DNA templates. These systems hold great promise for modular and on-demand bioproduction of valuable small molecules in resource-limited settings but are constrained in their long-term stability, reusability, and deployability. In this work, we demonstrate that multiple cell-free expressed enzymes can be co-immobilized in biocompatible hydrogels made from poly(ethylene glycol) diacrylate (PEGDA) with added glycerol for enhanced gel integrity.
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