Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Liquid-liquid phase separation (LLPS)-driven membraneless organelles (MLOs) have been employed to enhance metabolic efficiency in various microbial cell factories. However, their application in the industrial bacterium has not been explored. Here, we report the formation of liquid protein condensates in using the RGG domain of LAF-1. We optimized conditions for condensate formation, including the pre-induction period, inducer concentration, and cultivation temperature. Using the indigoidine biosynthesis pathway as a model, we demonstrated that LLPS-mediated MLOs enhanced indigoidine production. Furthermore, we applied these MLOs to modulate the toxicity of antimicrobial peptides (AMPs) to host cells, facilitating the expression of AMPs, including melittin and lactoferricin B. These findings provide insights into MLOs engineering in and suggest broader applications of LLPS-mediated systems in industrial biotechnology.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395988 | PMC |
| http://dx.doi.org/10.1016/j.synbio.2025.08.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Corrigendum to "Exploring mechanisms of resistance to fludioxonil in Colletotrichum fructicola" [Pesticide Biochemistry and Physiology 208 (2025) 106284].
Pestic Biochem Physiol
November 2025
National Key Laboratory for Germplasm Innovation Utilization of Horticultural Crops, and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
Endogenous YAP/TAZ partitioning in TEAD condensates orchestrates the Hippo response.
Mol Cell
August 2025
Lingang Laboratory, Shanghai 200031, China. Electronic address:
YAP/TAZ are transcriptional co-activators that pair with transcription factor TEA/ATTS domains (TEADs) for modulating the Hippo pathway. Previous works propose the potential role of YAP/TAZ phase separation for transcriptional activation, yet the biomolecular basis of endogenous YAP/TAZ-TEAD condensates remains unclear. Here, we dissect their endogenous morphology, revealing that YAP/TAZ are client proteins recruited to TEAD condensates in various human cell lines.
View Article and Find Full Text PDFUnveiling the role of biomolecular condensates in cellular function and cancer.
Adv Biol Regul
September 2025
Laboratory of Cancer Cell Architecture, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic. Electronic address:
Biomolecular condensates (BMCs) are membrane-less organelles formed through liquid-liquid phase separation, primarily driven by multivalent interactions between scaffold and client molecules. These dynamic compartments enable cells to spatially and temporally organize biochemical reactions by locally concentrating specific biomolecules, thereby enhancing the frequency of productive molecular interactions and increasing reaction rates. BMCs are integral to normal cellular physiology, with well-characterized examples including the nucleolus and Cajal bodies.
View Article and Find Full Text PDFChromatin-associated condensates as an inspiration for the system architecture of future DNA computers.
Ann N Y Acad Sci
September 2025
Institute of Biological and Chemical Systems, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
The genome stores and processes approximately 1.5 gigabytes of encoded information. In this article, we propose that the eukaryotic genome and its adaptable three-dimensional packing in the form of chromatin offer a valuable template for the system architecture of DNA-based digital computers.
View Article and Find Full Text PDFMask protein keeps NF-κB precursor inactive by inducing a closed conformation and isolating it in biomolecular condensates.
Cell Rep
September 2025
State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China. Electronic address:
Nuclear factor κB (NF-κB) family transcription factors are critical for innate immune responses across a variety of organisms and are frequently dysregulated in diseases. Understanding their homeostatic regulation is essential for developing therapeutic strategies. Relish, a Drosophila homolog of mammalian NF-κB precursors, provides a valuable model for studying these processes.
View Article and Find Full Text PDF