Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Heterologous expression of biosynthetic gene clusters (BGCs) is a powerful strategy for natural product (NP) discovery, yet achieving consistent expression across microbial hosts remains challenging. Here, we developed cross-phyla vector systems enabling the expression of BGCs from cyanobacteria and other bacterial origins in Gram-negative , Gram-positive , and two model cyanobacterial strains including unicellular PCC 6803 and filamentous sp. PCC 7120. Following validation using constitutive and inducible expression of the enhanced yellow fluorescent protein (eYFP), we applied these vectors to express the shinorine and violacein BGCs in all four hosts. Promoter tuning, substrate feeding, BGC refactoring, and inducible control enhanced NP production and mitigated host toxicity. Notably, we demonstrated that can serve as a chassis for cyanobacterial NP BGC expression. Our results provide versatile expression platforms for probing BGC function and accelerating natural product discovery from diverse cyanobacterial and other bacterial lineages.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acssynbio.5c00390 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Action at a distance: The remarkable coupling of CO uptake to electron transfer in specialized cyanobacterial NDH-1 complexes.
Proc Natl Acad Sci U S A
September 2025
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078.
Cyanobacteria achieve highly efficient photosynthesis using a CO-concentrating mechanism relying on specialized Type I (NDH-1) complexes. Among these, NDH-1 and NDH-1 catalyze redox-coupled hydration of CO to bicarbonate, supporting carbon fixation in carboxysomes. The mechanism of coupling electron transfer to CO-hydration by these variant NDH-1 complexes remains unknown.
View Article and Find Full Text PDFMutations in the circadian cycle drive adaptive plasticity in cyanobacteria.
Proc Natl Acad Sci U S A
September 2025
Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-Consejo Superior de Investigaciones Científicas, Santander, Cantabria 39011, Spain.
Circadian clocks allow organisms to anticipate daily fluctuations in light and temperature, but how this anticipatory role promotes adaptation to different environments remains poorly understood. Here, we subjected the cyanobacterium PCC 7942 to a long-term evolution experiment under high light, high temperature, and elevated CO levels. After 1,200 generations, we obtained a strain exhibiting a 600% increase in growth rate.
View Article and Find Full Text PDFMultichassis Expression of Cyanobacterial and Other Bacterial Biosynthetic Gene Clusters.
ACS Synth Biol
September 2025
Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development (CNPD3), University of Florida, Gainesville, Florida 32610, United States.
Heterologous expression of biosynthetic gene clusters (BGCs) is a powerful strategy for natural product (NP) discovery, yet achieving consistent expression across microbial hosts remains challenging. Here, we developed cross-phyla vector systems enabling the expression of BGCs from cyanobacteria and other bacterial origins in Gram-negative , Gram-positive , and two model cyanobacterial strains including unicellular PCC 6803 and filamentous sp. PCC 7120.
View Article and Find Full Text PDFOptimising CO level and light quality for enhanced whole-cell biotransformation reactions in Synechocystis sp. PCC 6803.
Microb Cell Fact
August 2025
Molecular Plant Biology Unit, Department of Life Technologies, University of Turku, Turku, Finland.
Cyanobacteria are emerging as a promising platform for whole-cell biotransformation, harnessing solar energy to drive biocatalytic reactions through recombinant enzymes. However, optimisation remains challenging due to the complexity of the cyanobacterial metabolism and the regulatory framework in which heterologous enzymes operate. While many enzymes have been deployed for light-driven whole-cell biotransformations, the different experimental conditions used between studies make direct comparison and systematic improvement difficult.
View Article and Find Full Text PDFA family of tubular pili from harmful algal bloom forming cyanobacterium Microcystis aeruginosa.
Nat Commun
August 2025
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA.
Cyanobacteria are vital photosynthetic prokaryotes, but some form harmful algal blooms (cyanoHABs) that disrupt ecosystems and produce toxins. The mechanisms by which these blooms form have yet to be fully understood, particularly the role of extracellular components. Here, we present a 2.
View Article and Find Full Text PDF