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Proteins are integral components of root exudates that mediate plant-microbe interactions, nutrient mobilization, and stress responses. Despite their importance, our understanding of their composition, regulation, and function is limited. Here, we summarize recent advances on root-exuded proteins, highlight pivotal outstanding questions, and propose future research directions.
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http://dx.doi.org/10.1016/j.tplants.2025.07.014 | DOI Listing |
Genome Biol
September 2025
Department of Biology, Plant-Microbe Interactions, Science for Life, Utrecht University, Utrecht, 3584CH, The Netherlands.
Background: Plant roots release root exudates to attract microbes that form root communities, which in turn promote plant health and growth. Root community assembly arises from millions of interactions between microbes and the plant, leading to robust and stable microbial networks. To manage the complexity of natural root microbiomes for research purposes, scientists have developed reductionist approaches using synthetic microbial inocula (SynComs).
View Article and Find Full Text PDFEcotoxicol Environ Saf
September 2025
Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Subtropic Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China. Ele
Seven plant growth-promoting bacteria (PGPB) were isolated from extracts of surface-sterilized Sedum alfredii Hance. Among the seven isolates, the strain SaRB5 identified as Stenotrophomonas maltophilia through 16S rDNA sequence analysis, exhibited highest levels of heavy metal resistance and plant growth-promoting traits. SaRB5 tolerated high concentrations of cadmium (Cd) (1.
View Article and Find Full Text PDFPlanta
September 2025
Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 3603, Lemesos, Cyprus.
Cypriot tomato landraces exhibit partial resistance to Fusarium wilt through distinct jasmonic and salicylic acid-mediated immune responses, offering promising genetic resources for breeding durable tomato cultivars. Fusarium wilt, caused by Fusarium oxysporum f. sp.
View Article and Find Full Text PDFPLoS Biol
September 2025
Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America.
Inter-laboratory replicability is crucial yet challenging in microbiome research. Leveraging microbiomes to promote soil health and plant growth requires understanding underlying molecular mechanisms using reproducible experimental systems. In a global collaborative effort involving five laboratories, we aimed to help advance reproducibility in microbiome studies by testing our ability to replicate synthetic community assembly experiments.
View Article and Find Full Text PDFPlant Physiol
September 2025
Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht 3508 TB, the Netherlands.
The increasing demand for sustainable agricultural practices has driven a renewed interest in plant-microbiome interactions as a basis for the next "green revolution." Central to these interactions are root-derived metabolites that act as mediators of microbial recruitment and function. Plants exude a chemically diverse array of compounds that influence the assembly, composition, and stability of the root microbiome.
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