Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Macrophage polarization plays a crucial role in the processes of inflammation, angiogenesis, and wound healing. N6-methyladenosine (mA) RNA modification has been widely recognized as an abundant modification that regulates RNA expression. This work aimed to investigate the function of mA modified Socs1 in skin wound healing.
Methods: A full-thickness skin wounds mouse model was established and treated with Socs1 overexpression. The wound healing process and the histological changes of skin tissues were detected. Ana-1 macrophages were treated with lipopolysaccharide (LPS) to mimic the inflammatory environment during the wound healing process. The macrophage polarization was detected by immunofluorescence staining of specific biomarkers and production of inflammatory factors was measured using ELISA kits. Angiogenesis and fibroblast proliferation and migration were measured by the co-culture system of Ana-1 with dermal microvascular endothelial cells (DMECs) or dermal fibroblasts (DFs). The mA modification of Socs1 mRNA was measured by mA mRNA immunoprecipitation.
Results: Socs1 expression was upregulated during wound healing process and M2 polarization of macrophages. Socs1 overexpression accelerated mouse skin wound healing and enhanced the formation of granulation tissue in wound tissues. Co-culture with Socs1-overexpressed macrophages increased angiogenesis of DMECs and enhanced the viability and migration of DFs. METTL14 regulates Socs1 expression in Ana-1 cells and increased the mA methylation of Socs1 mRNA by recruiting YTHDF1.
Conclusion: Socs1 regulates the M2 macrophages polarization and accelerates wound healing, which is modulated by METTL14-mediated mA modification of Socs1 mRNA through YTHDF1 recruitment in macrophages.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381997 | PMC |
| http://dx.doi.org/10.1186/s40001-025-03056-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: Pressure injuries are common, difficult to manage, and carry a high economic burden. They are challenging to physicians and a burden to society.
Case Report: An 89-year-old male, who had previously undergone internal fixation with screws and rods for a right intertrochanteric fracture, developed a deep circular open ulcer measuring 11 cm × 7.
Pulsed electromagnetic field stimulation therapy for Charcot foot ulcer: a randomized controlled trial.
Wounds
August 2025
Faculty of Physical Therapy, Cairo University, Cairo, Giza, Egypt.
Background: Charcot foot is a debilitating complication of peripheral neuropathy and is primarily associated with diabetes, leading to structural damage, ulceration, and osteomyelitis. Pulsed electromagnetic field (PEMF) therapy is a promising treatment modality for wound healing and bone metabolism.
Objective: To evaluate the efficacy of PEMF therapy in promoting bone growth and ulcer healing in patients with Charcot foot ulcers.
Use of a novel zipper device for wound closure of cutaneous abscesses in pediatric outpatients: a case series.
Wounds
August 2025
Department of Day Surgery, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorder, Chongqing, China; China International Science and Technology Coopera
Background: Current management of pediatric cutaneous abscesses involves either spontaneous healing by secondary intention or suturing through tertiary intention, which are often lengthy processes that cause discomfort and distress among children. As it is noninvasive and simple, a novel zipper device is widely used for the primary wound closure of surgical incisions.
Objective: To describe the effectiveness of novel zipper device use for pediatric cutaneous abscess wound closure in an outpatient context.
Background: This retrospective analysis is a derivative cohort study based on a prior retrospective investigation by this author group.
Objective: To assess the effect of the number of cellular and/or tissue-based product (CTP) applications on healing outcomes and wound area reduction (WAR) rates in patients with chronic wounds of multiple etiologies.
Methods: Data from a multicenter private wound care practice electronic health record database were analyzed for Medicare patients receiving CTPs from January 2018 through December 2023.
The trial design of the concurrent optical and magnetic stimulation (COMS) therapy study for refractory diabetic foot ulcers (MAVERICKS): a multicenter, randomized, sham-controlled, double-blind investigational device exemption clinical study.
Wounds
August 2025
Department of Nursing, Federal University of Ceará, Ceará, Brazil.
Background: Diabetic foot ulcers (DFUs) are a major clinical challenge, particularly among patients with refractory ulcers, that often lead to severe complications such as infection, amputation, and high mortality. Innovations supported by strong clinical evidence have the potential to improve healing outcomes, enhance quality of life, and reduce the economic burden on individuals and health care systems.
Objective: To describe the design of the concurrent optical and magnetic stimulation (COMS) therapy Investigational Device Exemption (IDE) study for refractory DFUs (MAVERICKS) trial.