Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
COL4A1, a key component of the basement membrane, has been increasingly implicated in tumor progression, yet its role in colon cancer remains incompletely understood. In this study, we conducted a comprehensive integrative analysis using transcriptomic data from the TCGA-COAD cohort, combined with functional validation in colon cancer cell lines. Gene set enrichment analysis (GSEA) revealed that high COL4A1 expression was associated with oncogenic pathways including epithelial-mesenchymal transition (EMT), KRAS signaling, and inflammatory responses. Immune infiltration analysis indicated that COL4A1 expression negatively correlated with CD8 T cell infiltration but positively correlated with macrophage subtypes. Immunophenoscore (IPS) analysis further revealed that tumors with high COL4A1 expression exhibited significantly higher IPS values, suggesting altered immunogenicity. Functional assays demonstrated that COL4A1 knockdown reduced cell proliferation, migration, and invasion in vitro. Co-expression analysis of EMT markers showed strong positive correlations between COL4A1 and mesenchymal genes such as VIM, ZEB1, SNAI1, and FN1, supporting its role in EMT-like phenotypes. Collectively, our findings suggest that COL4A1 may serve as a prognostic biomarker and contributor to tumor progression and immune remodeling in colon cancer.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381163 | PMC |
| http://dx.doi.org/10.1038/s41598-025-17230-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Perforation that Occurred Right After Sigmoid Colon Cancer Stenting.
Dig Dis Sci
September 2025
Department of Gastrointestinal Surgery, Meizhou People's Hospital, Meizhou Academy of Medical Sciences, No. 63, Xinfeng Road, Meijiang District, Meizhou, 514031, Guangdong, China.
Prostate sparing radical cystectomy and neobladder: institutional long-standing qualified consideration.
J Egypt Natl Canc Inst
September 2025
National Cancer Institute of Cairo University, Giza, Egypt.
Objectives: To balance the extended functional urinary voiding and morbidity outcomes amid Ileal W and Y-shaped contrasted to spherical ileocoecal (IC) orthotopic bladders subsequent prostate-sparing radical cystectomy (PRC) versus standard radical cystoprostatectomy (RC).
Material And Methods: Two hundred eight male bladder cancer patients were grouped into 98 RC followed by 43-W, 31-Y, and 23-IC in comparison to 110 PRC followed by 35-W, 37-Y, and 38-IC. The functional voiding outcomes were determined by detailed patients' interview and urodynamic studies (UDS).
LDH-chitosan bionanocomposites for oncologic applications: A refreshing perspective on the mutual influence through intermolecular forces toward controlled morphology and dispersion.
Int J Biol Macromol
September 2025
Nanotechnology Laboratory, TRANSCEND Research Center, Regional Institute of Oncology, 2-4 General Henri Mathias Berthelot Street, 700483, Iași, Romania; Faculty of Chemistry, Al. I. Cuza University, 11- Carol I Bvd., 700506, Iasi, Romania. Electronic address:
This contribution discusses the design of bionanocomposites based on chitosan and MgAl layered double hydroxides (LDH) for cancer therapy. Compared to other studies, our approach was to pre-adsorb the metal chloride precursors of LDH on chitosan while the solution of metal precursors with and without H provided the acidic environment for polymer dissolution. The structure, morphology and chemical composition of the bionanocomposites were characterized by XRD, FTIR, TG, etc.
View Article and Find Full Text PDFNK cells limit the synergistic anti-tumor effect of PD-1 inhibition and βγ-biased IL-2.
Int J Biol Macromol
September 2025
Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, 213003, China; Jiangsu Engineering Research Center for Tumor Immunotherapy, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, 213003, China; Institute of Cell The
Despite its potential as a cancer immunotherapy, wild-type IL-2 is limited by dose-limiting toxicities, including vascular leak syndrome, and its strong activation of regulatory T cells (Tregs), which dampens anti-tumor immunity. These drawbacks are largely driven by IL-2's binding to IL-2Rα, and avoiding this interaction can reduce IL-2-associated toxicities, although it cannot completely eliminate them. To overcome these limitations, βγ-biased IL-2 variants (Non-α-IL-2) have been developed to selectively activate effector T and NK cells.
View Article and Find Full Text PDFSafety of artificial intelligence-assisted optical diagnosis for leaving colorectal polyps in situ during colonoscopy (PRACTICE): a non-inferiority, randomised controlled trial.
Lancet Gastroenterol Hepatol
October 2025
Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Italy; Endoscopy Unit, IRCCS Humanitas Research Hospital, Rozzano, Italy.
Background: Guidelines recommend leaving in situ rectosigmoid polyps diagnosed during colonoscopy that are 5 mm or smaller if the endoscopist optically predicts them to be non-neoplastic. However, no randomised controlled trial has been done to examine the efficacy and safety of this strategy.
Methods: This open-label, multicentre, non-inferiority, randomised controlled trial enrolled adults age 18 years or older undergoing colonoscopy for screening, surveillance, or clinical indications across four Italian centres.