ds, a Potential RNA Biopesticide for Controlling by Targeting the Synthesis of Sex Pheromones.

J Agric Food Chem

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi Key Laboratory of Biotechnology, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.

Published: September 2025


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Article Abstract

(Hübner) is an important polyphagous agricultural pest that poses a serious threat to global agricultural production. We characterized the pheromone biosynthesis-activating neuropeptide receptor (PBANR) in and elucidated its critical role in sex pheromone biosynthesis and reproduction. Bioinformatic analysis and functional assays confirmed that PBANR is a rhodopsin-like G protein-coupled receptor (GPCR). Expression of peaked in female adults and was essential for regulating intracellular calcium homeostasis and sex pheromone production. RNA interference (RNAi)-mediated silencing of significantly reduced the levels of key sex pheromones (Z9,E12-14:Ac and Z9-14:Ac), suppressed mating frequency, and decreased egg production. These findings demonstrate that -targeting dsRNA may serve as a potential RNAi-based biopesticide to disrupt pheromone-mediated reproduction in .

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http://dx.doi.org/10.1021/acs.jafc.5c06424DOI Listing

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