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We report a case of culture-negative infective endocarditis in a 64-year-old hemodialysis patient who presented with fever and dyspnea. Despite empirical antibiotic therapy and negative blood cultures, transesophageal echocardiography later revealed aortic valve vegetation and destruction. The patient developed cardiogenic shock and died before surgery. This case illustrates the diagnostic challenges of culture-negative infective endocarditis, especially in high-risk populations. Early blood culture collection and continuous reassessment of surgical timing are crucial to improve outcomes.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12358075 | PMC |
http://dx.doi.org/10.7759/cureus.88226 | DOI Listing |
Infect Drug Resist
September 2025
School of Basic Medical Sciences, Henan University, Kaifeng, People's Republic of China.
Background: This study evaluated the applicability of histopathology, culture, and Metagenomic next-generation sequencing (mNGS) in diagnosing periprosthetic joint infection (PJI).
Methods: In this prospective trial, 215 consecutive patients with suspected knee PJI were enrolled. Tissue specimens were aseptically collected and processed for histopathological analysis, culture, and mNGS.
Infect Drug Resist
August 2025
Department of Clinical Laboratory, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, People's Republic of China.
Background: commonly colonizes the genitourinary tract and primarily affects immunocompromised individuals. It is mostly confined to localized infections, with bloodstream dissemination being rare. Because of its fastidious nutritional requirements, the organism is seldom recovered by routine blood culture, and the absence of a cell wall renders it intrinsically resistant to many first-line antimicrobials.
View Article and Find Full Text PDFCureus
August 2025
Neurological Surgery, Punjab Institute of Neurosciences, Lahore, PAK.
Brain abscesses are life-threatening infections, predominantly caused by anaerobic organisms. The role of oropharyngeal microbiota, presence in dental plaque biofilms, and hematogenous spread is established in the literature. However, due to its rare occurrence, limited literature is available on its management.
View Article and Find Full Text PDFLancet Infect Dis
September 2025
The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Background: Based on results from preclinical and clinical studies, a five-drug combination of isoniazid, rifapentine, pyrazinamide, ethambutol, and clofazimine was identified with treatment shortening potential for drug-susceptible tuberculosis; the Clo-Fast trial aimed to determine the efficacy and safety of this regimen. We compared 3 months of isoniazid, rifapentine, pyrazinamide, ethambutol, and clofazimine, administered with a clofazimine loading dose, to the standard 6 month regimen of isoniazid, rifampicin, pyrazinamide, and ethambutol in drug-susceptible tuberculosis.
Methods: Clo-Fast was a phase 2c open-label trial recruiting participants at six sites in five countries.
J Dairy Sci
September 2025
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691. Electronic address:
Intramammary infections are common in nonlactating dairy cattle and have been shown to disrupt mammary tissue architecture in nonpregnant heifers. However, their effect on mammary development during pregnancy remains unclear. This study assessed the effects of IMI on mammary gland development in pregnant dairy heifers during late gestation.
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