Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Oral squamous cell carcinoma (OSCC) is the most prevalent form of oral cancer, with about one-third of patients facing life-threatening and recurrent cases. Certain pathobionts in the oral microbiome contribute to the rise of inflammation and are directly involved in carcinogenesis. Analysis of the microbial profiles in OSCC patients reveals significant differences compared to healthy individuals. This study investigates the microbial composition, microRNA (miRNA) expression, and their related genes for the first time in Iranian patients diagnosed with OSCC.
Methods: The relative presence of Fusobacterium nucleatum, Porphyromonas gingivalis, Fusobacterium periodonticum, and Prevotella intermedia was assessed in both tumor and adjacent normal tissues using Real-time PCR on 20 pairs of fresh-frozen OSCC samples. The expression levels of four miRNAs (miR-21, miR-31, miR-26a, and miR-29a) and five target genes were investigated. Eventually, any potential association between these bacteria's presence and miRNAs' expression was analyzed.
Results: P. gingivalis and F. nucleatum were significantly more abundant in tumor samples than in the control samples. However, the other two bacteria did not show significant differences between the tumor and control samples. The relative expression levels of both oncomiRs were significantly higher in tumors compared to control samples. The analysis indicates a significant association between the presence of F. nucleatum and miR-21 expression. Additionally, a significant association was identified between P. gingivalis and the expression of miR-31. In addition, LOXL2 was the only miRNA-related gene that showed a substantial increase in tumor tissue compared to the control.
Conclusion: There is a significant association between the presence of F. nucleatum and miR-21; a relationship has been reported in colorectal cancer and is now observed in OSCC, and the expression of miR-31 was significantly correlated with P. gingivalis. Further research is needed to explore the prevalence and potential mechanisms of pathobionts and miRNA expression in OSCC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11033-025-10926-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Gene dysregulation impairs placental angiogenesis in allogeneic pig pregnancies.
Anim Reprod Sci
September 2025
Department of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping SE-58185, Sweden.
Embryo transfer (ET) is a valuable reproductive technology in pigs, albeit its efficiency remains significantly lower than that of natural mating or artificial insemination (AI), owing to high embryonic death rates. Critical for embryo survival and pregnancy success is the placenta, which supports conceptus development through nutrient exchange, hormone production, and immune modulation. Alterations in placental development and function may therefore underlie the reduced efficiency of ET.
View Article and Find Full Text PDFFlorida red scale Chrysomphalus aonidum (Hemiptera: Diaspididae) and parasitoid Aphytis melinus (Hymenoptera: Aphelinidae) colonization of Citrus Under Protective Screen.
J Econ Entomol
September 2025
Department of Entomology and Nematology, Southwest Florida Research and Education Center (SWFREC), University of Florida/IFAS, Immokalee, FL, USA.
The Citrus Under Protective Screen is a novel production system implemented to grow citrus free of huanglongbing disease vectored by Asian citrus psyllid, Diaphorina citri. Other significant pests such as mites, scales, thrips, mealybugs, and leafminers, as well as parasitoids and small predators, have been identified from Citrus Under Protective Screen and require management. Chrysomphalus aonidum (L.
View Article and Find Full Text PDFControlling collagen I orientation on polyetheretherketone implants to improve epithelial sealing.
ACS Biomater Sci Eng
September 2025
Materials Engineering, McGill university, Montreal H3A0C5, Canada.
Transcutaneous devices such as dental implants frequently fail due to infections at their interfaces with epithelial tissues. These infections are facilitated by the lack of integration between the devices and the surrounding soft tissues. This study aims to improve epithelial integration through surface modification of a transcutaneous implant material (polyetheretherketone (PEEK)).
View Article and Find Full Text PDFLong-Term Open-Label Study Evaluating Oral Miglustat Treatment in Patients With Neuronal Ceroid Lipofuscinosis Type 3.
Neurology
October 2025
Neurology, Epilepsy and Movement Disorders Unit, Bambino Gesù Children's Hospital, IRCCS, Full Member of European Reference Network on Rare and Complex Epilepsies - EpiCARE, Rome, Italy.
Objectives: Neuronal ceroid lipofuscinosis type 3 (CLN3) is a rare lysosomal storage disorder characterized by progressive neurodegeneration. No disease-modifying treatments are currently available. Miglustat, a substrate reduction therapy, has shown preclinical efficacy in CLN3 models (conference abstract).
View Article and Find Full Text PDFMonocyte Anisocytosis Can Discriminate Between Sepsis and Sterile Inflammation, but not Mortality, in Critically Ill Surgical/Trauma Patients: A Secondary Prospective Analysis.
Crit Care Explor
September 2025
Department of Biostatistics, University of Florida Colleges of Medicine and Public Health and Health Professions, Gainesville, FL.
Objectives Background: Monocyte anisocytosis (monocyte distribution width [MDW]) has been previously validated to predict sepsis and outcome in patients presenting in the emergency department and mixed-population ICUs. Determining sepsis in a critically ill surgical/trauma population is often difficult due to concomitant inflammation and stress. We examined whether MDW could identify sepsis among patients admitted to a surgical/trauma ICU and predict clinical outcome.
View Article and Find Full Text PDF