Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The long non-coding RNAs (lncRNAs) can bind to transcription factors or RNA-binding proteins to play important regulatory roles in muscle growth and development. This study investigated the functional role of the LNC_004268 in sheep myoblast proliferation and differentiation, as well as its interaction with the RNA-binding protein hnRNPK and the downstream target gene CNOT2. LNC_004268 shows higher expression in the longissimus dorsi muscle of Small-tailed Han sheep (STH) compared to Sunite sheep (SNT) and is primarily localized in the nucleus. Functionally, LNC_004268 inhibits myoblast proliferation while promoting differentiation and myotube formation. Mechanistically, LNC_004268 binds hnRNPK, stabilizing CNOT2 mRNA. Actinomycin D (ACD) assays confirmed that LNC_004268 or hnRNPK overexpression delays CNOT2 mRNA decay, with combined expression further enhancing stability. Notably, CNOT2 functionally recapitulates LNC_004268's effects on myogenesis. Collectively, our study demonstrates that LNC_004268 regulates myoblast proliferation and differentiation by stabilizing CNOT2 mRNA via hnRNPK interaction, advancing understanding of lncRNA mediated muscle development regulation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2025.146625 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
X-Linked Hypophosphatemia: Role of Fibroblast Growth Factor 23 on Human Skeletal Muscle-Derived Cells.
Calcif Tissue Int
September 2025
FirmoLab, Fondazione F.I.R.M.O. Onlus and Stabilimento Chimico Farmaceutico Militare (SCFM), 50141, Florence, Italy.
X-linked hypophosphatemia (XLH) is a rare and progressive disease, due to inactivating mutations in the phosphate-regulating endopeptidase homolog X-linked (PHEX) gene. These pathogenic variants result in elevated circulating levels of fibroblast growth factor 23 (FGF23), responsible for the main clinical manifestations of XLH, such as hypophosphatemia, skeletal deformities, and mineralization defects. However, XLH also involves muscular disorders (muscle weakness, pain, reduced muscle density, peak strength, and power).
View Article and Find Full Text PDFYolk extract-derived vitellogenin 2 ameliorates muscle atrophy in mice the PI3K/AKT/mTOR pathway.
Food Funct
September 2025
College of Veterinary Medicine, Shanxi Agricultural University, Taiyuan, Shanxi 030801, China.
Eggs play an important role in skeletal muscle development, but their active components are unknown. The aim of this study was to investigate the effect of yolk extract-derived vitellogenin 2 on dexamethasone (DEX)- and cancer cachexia (CC)-induced skeletal muscle atrophy. We used iTRAQ to detect the changes in protein expression between fertilized egg yolk extract (FEYE) and unfertilized egg yolk extract (UEYE).
View Article and Find Full Text PDFExtracellular vesicles-delivered circDB promotes ischemic muscle repair through the miR-34a/USP7/Notch1 signaling pathway.
Regen Ther
December 2025
Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Suzhou, Jiangsu, 215123, PR China.
Introduction: The incidence of lower limb ischemic diseases has been rising steadily in recent years, often leading to severe outcomes such as limb amputation. Given the limited availability of effective treatments, there is a critical need for novel therapeutic strategies. This study explores the reparative role and underlying mechanisms of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UMSC-EVs) in promoting ischemic hindlimb recovery through the delivery of circular RNA circDB.
View Article and Find Full Text PDFRPS27L Enhances Myogenesis and Muscle Mass by Targeting IGF1 Through Liquid-Liquid Phase Separation.
Adv Sci (Weinh)
August 2025
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China.
RNA-binding proteins (RBPs) play a pivotal role in post-transcriptional regulation of gene expression, critically influencing skeletal myogenesis, muscle growth, and regeneration. Despite the recent identification of RBP Rps27l (ribosomal protein S27-like) as a regulator affecting myogenic proliferation and differentiation, its functions and regulatory mechanisms in skeletal muscle development remain largely unknown. In this study, it is observed that muscle-specific Rps27l knock-in (M─KI) mice exhibit significantly increased muscle mass, enlarged myofiber size, a higher proportion of fast-twitch myofibers, and enhanced muscle regeneration capabilities compared to wild-type controls.
View Article and Find Full Text PDFTransplantation of Cultured Myoblasts Into Intact Skeletal Muscle and Analysis of Muscle Contraction Force in Mice Model.
Bio Protoc
August 2025
Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji-City, Tokyo, Japan.
Cell transplantation is a promising strategy for treating age-related muscle atrophy, but its critical application remains limited. Cultured myoblasts, unlike freshly isolated muscle stem cells, show poor engraftment efficiency and fail to contribute effectively to muscle regeneration. Moreover, successful engraftment generally requires prior muscle injury, as skeletal muscle regeneration is typically triggered by a damaged microenvironment.
View Article and Find Full Text PDF