Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objective: Various vestibular pathology models have been developed to elucidate the underlying mechanisms of vestibular diseases; however, there is a notable absence of minimally invasive mouse model of vestibular dysfunction. In this study, we established a novel model and conducted a comprehensive evaluation of vestibular function alongside various behavioral assessments.
Methods: We developed a surgical procedure involving canalostomy followed by injection of absolute ethanol into the semicircular canal of mice. Histological analysis and auditory brainstem response (ABR) testing were conducted to confirm vestibular and cochlear damage. Vestibular dysfunction was assessed using a syndrome scoring system, the horizontal angular vestibulo-ocular reflex (hVOR) test, and the rotarod test. Additionally, home-cage behavior was recorded to evaluate spontaneous behavioral changes.
Results: The surgical procedure resulted in significant, irreversible damage to the vestibulocochlear structures at the pre-ganglionic level. The model exhibited severe vestibular symptoms, including acute weight loss, postural deviation, reduced locomotor ability, and impaired hVOR function. Static vestibular compensation was observed within 14 days post-surgery, whereas dynamic compensation progressed more gradually. Home-cage behavior monitoring revealed increased walking and turning activity, accompanied by a reduction in other dynamic and exploratory behaviors (sniffing, rearing, and hanging) compared to sham controls. In contrast, basic behaviors related to survival (sleeping, drinking, eating, and grooming) were largely preserved.
Conclusion: In this study, we successfully established a novel mouse model of acute vestibular dysfunction via semicircular canal injection of absolute ethanol. This model is minimally invasive, reproducible, and exhibits consistent behavioral phenotypes, making it a valuable tool for studying peripheral vestibular disorders and their compensatory mechanisms.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.heares.2025.109366 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring Heterogeneity in Vestibular Migraine Using Individualized Differential Structural Covariance Network Analysis.
CNS Neurosci Ther
September 2025
Department of Radiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Background: The high heterogeneity in vestibular migraine (VM) complicates understanding its precise pathophysiological mechanisms and identifying potential biomarkers. This study investigated the heterogeneity in VM using a newly proposed method called Individualized Differential Structural Covariance Network (IDSCN) analysis.
Methods: Structural T1-weighted MRI scans were performed on 55 patients with VM and 65 healthy controls, and an IDSCN was constructed for each patient.
Rethinking positional nystagmus: beyond BPPV.
J Neurol
September 2025
SENSE Research Unit, Department of Clinical and Movements Neurosciences, UCL Institute of Neurology, 33 Queen Square, London, WC1N 3BG, UK.
Positional nystagmus is a frequently encountered yet often underappreciated clinical sign that provides critical insights into vestibular and central nervous system function. For the general neurologist, recognising and correctly interpreting positional nystagmus can significantly impact diagnostic accuracy and guide appropriate management of common and complex dizziness presentations. The current diagnostic framework for positional nystagmus disproportionately favours BPPV, underestimates central positional nystagmus (CPN), and over-relies on imaging.
View Article and Find Full Text PDFAutonomic Plexuses of the Female Sexual Organs Travel Within Pubocervical and Rectovaginal [Endopelvic] Fascia: A Rationale for 'Nerve-Aware' Vaginal Prolapse Repair.
Clin Anat
September 2025
Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, Western University, London, Ontario, Canada.
The connective tissue support of female pelvic viscera-endopelvic fascia-has been studied in fetal and immunohistochemical models to demonstrate its relationship with the autonomic nerves of the female pelvis. Due to a paucity of literature examining the gross anatomical relationships between endopelvic fascia and autonomic nerves in adult female pelvises, it remains unknown whether defects in endopelvic fascia predisposing pelvic organ prolapse and/or manipulation of endopelvic fascia during prolapse repair may be the cause of prolapse-related pelvic pain and sexual dysfunction. Through the dissection of formalin-fixed hemipelvises (n = 10) the present study aimed to map the loci of the visceral branches of the inferior hypogastric plexus and associate them with endopelvic fascia of the female pelvis.
View Article and Find Full Text PDFQuantitative study of vestibular endolymphatic hydrops in Meniere's disease based on Three-Dimensional real Inversion Recovery (3D-real IR) sequence.
Eur J Radiol
August 2025
Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address:
Objective: To establish objective criteria for grading vestibular endolymphatic hydrops (EH) in Meniere's disease (MD) using 3D-real IR MRI, and investigate its clinical correlations.
Methods: Retrospective analysis of 155 MD patients (189 ears) included clinical data, audiovestibular assessments, and gadolinium-enhanced MRI. Vestibule volume (VV) and the volume of vestibular endolymph (EndV) were outlined on 3D-real IR sequence images, and the volume of vestibular endolymph was calculated by the vestibular endolymphatic ratio (ELR) to evaluate the degree of EH, analyze the correlation between the degree of EH and specific clinical features, hearing and vestibular function, and determine the optimal ELR value to differentiate each degree of effusion by using receiver operating characteristic (ROC) curve analysis.
Ocular Motor and Vestibular Profile in Spinocerebellar Ataxia Type 27B: Toward a Practical Bedside Diagnostic Framework.
Cerebellum
September 2025
Department of Neurology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Spinocerebellar ataxia type 27B (SCA27B), caused by GAA repeat expansions in FGF14, is an increasingly recognized form of late-onset cerebellar ataxia. However, early diagnosis remains challenging due to mild or absent cerebellar motor signs and often normal brain magnetic resonance imaging (MRI). Oculovestibular abnormalities, although prevalent, are frequently overlooked and not captured by standard clinical scales such as the Scale for the Assessment and Rating of Ataxia (SARA).
View Article and Find Full Text PDF