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Article Abstract
The effect of deproteinized Tremella olysaccharide (DTP) on the digestion of potato starch (PS) was investigated. DTP reduced the hydrolysis rate of PS in a concentration-dependent manner, with digestion rates of 76.99 %, 72.03 %, and 29.73 % after 180 min for 0.3, 0.6, and 1.2 mg/mL DTP, respectively. DTP delayed digestion by shifting the kinetics from single-phase to biphasic behavior. It significantly inhibited pancreatic α-amylase (IC = 5.76 mg/mL) by modifying the enzyme's secondary structure and reducing substrate binding. Additionally, DTP enhanced digesta viscosity, which may have contributed to the reduction in postprandial glucose release, thereby supporting its role as a potential hypoglycemic agent. However, DTP exhibited minimal glucose adsorption, binding only 1.2 % of 100 mM glucose at 1.2 mg/mL. These findings suggest that DTP reduces starch digestion primarily by inhibiting α-amylase activity and increasing digestive viscosity, providing insights into its hypoglycemic potential.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284542 | PMC |
| http://dx.doi.org/10.1016/j.fochx.2025.102759 | DOI Listing |
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