Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Omalizumab can provide clinical benefits to a fraction of moderate-to-severe asthma patients. However, the mechanisms of action of omalizumab have not been fully understood.
Objective: This study investigated whether omalizumab could affect the frequency and surface phenotypes of blood circulating dendritic cell (DC) and monocyte subsets, which could be associated with the mechanisms of action of omalizumab.
Methods: Longitudinal analyses of the frequency and surface phenotypes of DC and monocyte subsets in fresh whole blood of moderate-to-severe asthma patients (n = 45, 34 with response and 11 without response, from baseline to week 26) were performed by flow cytometry. Nonasthmatic subjects (n = 22) were also used as controls at baseline.
Results: Omalizumab decreased myeloid DC/plasmacytoid DC ratio by increasing the frequency of plasmacytoid DCs. In addition to the decrease of surface FcεRI expression, omalizumab also downregulated HLA-DR, CCR7, and costimulatory molecule expression on both myeloid DCs and plasmacytoid DCs. Omalizumab also decreased CCR7 and HLA-DR expression by monocyte subsets. Omalizumab-mediated reduction of surface CD88 expression on monocytes was associated with asthma symptoms.
Conclusion: This study provides new insight into omalizumab's mechanisms of action. Data from this study will help us understand the roles of serum IgE in shaping surface phenotypes of DCs and monocytes in asthma patients.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281943 | PMC |
| http://dx.doi.org/10.1016/j.jacig.2025.100523 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Escherichia coli strain O55 contains two cryptic plasmids that depend on each other to replicate.
Arch Microbiol
September 2025
División de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Zip Code 36050, Guanajuato, Mexico.
Plasmids are fundamental to molecular biology and biotechnology, playing a crucial role in bacterial evolution. Some plasmids are linked to complex cellular dynamics, including pathogenicity islands, antibiotic resistance, and gene mobilization. This study reports the isolation and sequencing of two cryptic plasmids with different electrophoretic mobilities from the Escherichia coli clinical isolate O55.
View Article and Find Full Text PDFUltra-low attachment surface enabling 3D co-culture of human B cells with CD40L-expressing stromal cells for mimicry of secondary lymphoid organs.
Biomater Sci
September 2025
Biotechnology Science and Engineering Program, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
B cells are critical components of the adaptive immune system that proliferate and differentiate within the secondary lymphoid organs upon recognition of antigens and engagement of T cells. Traditional two-dimensional (2D) cell cultures fall short of replicating the intricate structures and dynamic evolution of three-dimensional (3D) environments found in lymphoid organs, prompting the development of more physiologically pertinent models. Our approach employs -hexanoyl glycol chitosan (HGC) coated ultra-low attachment (ULA) lattice plates to cultivate a 3D co-culture of CD40L-expressing MS5 stromal cells and naïve B cells derived from the peripheral blood mononuclear cells (PBMCs) of healthy human donors.
View Article and Find Full Text PDF[Clinicopathological features and prognosis of aggressive natural killer-cell leukemia: an analysis of 27 cases].
Zhonghua Bing Li Xue Za Zhi
September 2025
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
To investigate the clinicopathological features, diagnosis, and prognosis of aggressive natural killer-cell leukemia (ANKL). A retrospective analysis was conducted on 27 ANKL patients treated at the First Affiliated Hospital of Nanjing Medical University from 2014 to 2024. Their clinical data, histomorphology, and immunophenotype were reviewed.
View Article and Find Full Text PDFThe immune receptor SLAMF5 regulates myeloid-cell mediated neuroinflammation in multiple sclerosis.
PLoS Biol
September 2025
Department of Systems Immunology, Weizmann Institute of Science, Rehovot, Israel.
Multiple sclerosis (MS) is a chronic neurological disorder characterized by demyelination of the central nervous system (CNS), leading to a broad spectrum of physical and cognitive impairments. Myeloid cells within the CNS, including microglia and border-associated macrophages, play a central role in the neuroinflammatory processes associated with MS. Activation of these cells contributes to the local inflammatory response and promotes the recruitment of additional immune cells into the CNS.
View Article and Find Full Text PDFA Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells.
J Vis Exp
August 2025
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology.
We present multimodal confocal Raman micro-spectroscopy (RS) and tomographic phase microscopy (TPM) for quick morpho-chemical phenotyping of human breast cancer cells (MDA-MB-231). Leveraging the non-perturbative nature of these advanced microscopy techniques, we captured detailed morpho-molecular data from living, label-free cells in their native physiological environment. Human bias-free data processing pipelines were developed to analyze hyperspectral Raman images (spanning Raman modes from 600 cm to 1800 cm, which uniquely characterize a wide range of molecular bonds and subcellular structures), as well as morphological data from three-dimensional refractive index tomograms (providing measurements of cell volume, surface area, footprint, and sphericity at nanometer resolution, alongside dry mass and density).
View Article and Find Full Text PDF