Distribution of Methylene Tetrahydrofolate Reductase Gene Polymorphisms in Women of Childbearing Age in Tai'an.

The objective was to explore the distribution characteristics of methylenetetrahydrofolate reductase 677C > T and 1298A > C gene polymorphisms among women of childbearing age in the Tai'an area. A total of 243 women of childbearing age, the age ranged from 20 to 46 years, with a mean age of 31.0 ± 5.1 years, attending the Eugenics Clinic of Tai'an City Central Hospital between January 2018 and April 2019 were selected. The 677C > T and 1298A > C gene polymorphisms were determined by fluorescence in situ hybridization. Among 243 women of childbearing age, the 677C > T genotype frequencies of CC, CT, and TT were 14.4%, 44.9%, and 40.7%, respectively, and the C and T Allele frequencies were 36.8% and 63.2%, respectively. The 1298A > C genotype frequencies of AA, AC, and CC were 77.0%, 21.0%, and 2.0%, respectively. The A and C allele frequencies were 87.4% and 12.6%, respectively. The proportions of CC/AA, CC/AC, CC/CC, CT/AA, CT/AC, TT/AA, and TT/AC were 6.6%, 5.8%, 2.0%, 30.5%, 14.4%, 39.9%, and 0.8%. The proportion of women at moderate or severe risk of folic acid metabolism reached 87.6%. The 677C > T gene mutation rate has obvious regional characteristics. The 677C > T and 1298A > C gene polymorphisms in Tai'an were obvious. About 87.6% of women of childbearing age were at medium or high risk of folic acid utilization.