Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Large-scale cellular production systems offer a significant and diverse benefit impacting the therapeutic (stem cell and vaccine production) and cellular agriculture (lab-grown meat) sectors. Producing desired cells at mass can improve production yield whilst reducing the environmental and ethical burden associated with industrialised agriculture and production of therapeutic goods. Many existing large-scale cultivation strategies of adherent cells leverage the use of microcarriers (MCs) within bioreactors. However, currently commercial MCs are not dissolvable and lack specificity for different cell types and bioprocessing contexts. In this work, we validate the effectiveness of customisable, polymeric MCs engineered to enhance cell growth and productivity. These MCs, which can be adjusted in terms of stiffness, surface charge, and size, maintain their structural integrity while offering precise property modifications. Under specific bioprocessing conditions, the custom MCs demonstrated significant improvements in cell productivity and sustainability compared to other commercial options. Our study (1) highlights how tailored substrate properties, particularly stiffness, can significantly impact cell yield and outcomes, and (2) suggests additional optimisations in surface charge and size that could further enhance MC technology. These advancements have the potential to improve large-scale cell and virus production efficiency, ultimately reducing the cost of production.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1088/1748-605X/adf1cd | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Amphiphilic Poly(SBMA--EGDEA) Coatings for Marine Antifouling: Insights into Design Criteria for Hydrophobic Monomers.
Biomacromolecules
September 2025
Department of Chemistry, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Marine biofouling poses significant economic and environmental challenges, highlighting the need for effective antifouling coatings. We report amphiphilic poly(SBMA--EGDEA) copolymer coatings that resist both marine diatom adhesion and sediment adsorption. The coatings were synthesized via surface-initiated ATRP and RAFT polymerization using hydrophilic sulfobetaine methacrylate (SBMA) and hydrophobic ethylene glycol dicyclopentenyl ether acrylate (EGDEA).
View Article and Find Full Text PDFHamiltonian Grid-Based QM/MM Method with Mean-Field Embedding for Simulating Arbitrary Slab Geometries.
J Chem Theory Comput
September 2025
Materials DX Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
The quantum mechanics/molecular mechanics (QM/MM) method is a powerful approach for investigating solid surfaces in contact with various types of media, since it allows for flexible modeling of complex interfaces while maintaining an all-atom representation. The mean-field QM/MM method is an average reaction field model within the QM/MM framework. The method addresses the challenges associated with the statistical sampling of interfacial atomic configurations of a medium and enables efficient calculation of free energies.
View Article and Find Full Text PDFProtein Deamidation Reduced Digestive Resistance and Amyloid Antigenicity of Soy Proteins via Depolymerization.
J Agric Food Chem
September 2025
Laboratory of Food Proteins and Colloids, School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
Soy protein remains a key component of plant-based food development, but its application is challenged by inherent allergenicity. Previous work identified that native amyloid-like protein aggregates in soy 7S globulin that resist gastrointestinal digestion and exhibit pronounced antigenicity. Herein, we demonstrate that protein deamidation significantly enhances proteolysis under an infant gastrointestinal digestion model, leading to ∼80 and 50% reductions in IgG- and IgE-binding capacities, respectively.
View Article and Find Full Text PDFProbing the Quantum Capacitance of Rydberg Transitions of Surface Electrons on Liquid Helium via Microwave Frequency Modulation.
Phys Rev Lett
August 2025
RIKEN Center for Quantum Computing, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
We present a method for probing the quantum capacitance associated with the Rydberg transition of surface electrons on liquid helium using radio-frequency (rf) reflectometry. Resonant microwave excitation of the Rydberg transition induces a redistribution of image charges on capacitively coupled electrodes, giving rise to a quantum capacitance originating from adiabatic state transitions and the finite curvature of the energy bands. By applying frequency-modulated resonant microwaves to drive the Rydberg transition, we systematically measured a capacitance sensitivity of 0.
View Article and Find Full Text PDFSurface-Morphology-Assisted Trapping of Strongly Coupled Electron-on-Neon Charge States.
Phys Rev Lett
August 2025
Washington University, Physics Department, Saint Louis, Missouri 63130, USA.
Single electrons confined to a free neon surface and manipulated through the circuit quantum electrodynamics architecture is a promising novel quantum computing platform. Understanding the exact physical nature of the electron-on-neon (eNe) charge states is important for realizing this platform's potential for quantum technologies. We investigate how resonator trench depth and substrate surface properties influence the formation of eNe charge states and their coupling to microwave resonators.
View Article and Find Full Text PDF