Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Recent innovations in extended in vitro culture (IVC) systems have revolutionized our understanding of human peri-implantation development. Building on foundational animal studies, refined protocols now support human embryo culture beyond the blastocyst stage, providing unprecedented access to previously elusive developmental events. These systems have yielded critical insights into early morphogenetic processes, lineage specification, and tissue organization, significantly advancing developmental biology. Here, we provide our current protocol for the extended culture of human embryos, followed by immunofluorescence for lineage markers of interest. Unveiling human peri-implantation development also promises to improve reproductive medicine, potentially addressing challenges related to implantation failure, chromosomal instability in embryos, as well as congenital disorders. Insights gained from this research may pave way for novel therapeutic approaches and advancements in medically assisted reproduction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/7651_2025_641 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Simulations of Implanted Glutamate Sensor Performance in Three Spatial Dimensions and Time Inform Sensor Design and Data Interpretation.
ACS Chem Neurosci
September 2025
Chemical and Biomolecular Engineering Dept, University of California, Los Angeles, Los Angeles, California 90095, United States.
Simulations in three dimensions and time provide guidance on implantable, electroenzymatic glutamate sensor design; relative placement in planar sensor arrays; feasibility of sensing synaptic release events; and interpretation of sensor data. Electroenzymatic sensors based on the immobilization of oxidases on microelectrodes have proven valuable for the monitoring of neurotransmitter signaling in deep brain structures; however, the complex extracellular milieu featuring slow diffusive mass transport makes rational sensor design and data interpretation challenging. Simulations show that miniaturization of the disk-shaped device size below a radius of ∼25 μm improves sensitivity, spatial resolution, and the accuracy of glutamate concentration measurements based on calibration factors determined .
View Article and Find Full Text PDFHSPiP and QbD oriented optimized nanocubosomes for ameliorated absorption of tolterodine tartrate: and evaluations.
Int J Pharm X
December 2025
School of Pharmaceutical Sciences, Lovely Professional University, Phagwada, Punjab, India.
The study explored HSPiP and QbD-(quality by design) enabled optimized cubosomes for sustained drug release, improved permeation, and enhanced oral bioavailability. OCUB1 (the optimized product) was characterized for size, zeta potential (ZP), thermal analysis, and surface roughness. drug release and hemolysis studies were carried out using a dialysis membrane and rat erythrocytes (4 % suspension), respectively.
View Article and Find Full Text PDFCollagen-elastin dermal scaffolds enhance tissue regeneration and reduce scarring in preclinical models.
Mater Today Bio
October 2025
Radboud University Medical Center, Research Institute for Medical Innovation, Department of Medical BioSciences, Geert Grooteplein 28, 6525 GA, Nijmegen, the Netherlands.
Severe scarring is an inevitable consequence of large full-thickness skin wounds, often leading to long-term complications that affect patients' well-being and necessitate extended medical interventions. While autologous split-thickness skin grafts remain the clinical standard for wound treatment, they frequently result in contractures, excessive scarring, and the need for additional corrective procedures. To address these challenges, bioengineered skin substitutes capable of promoting efficient healing while reducing complications are highly desirable.
View Article and Find Full Text PDFCereblon upregulation overcomes thalidomide resistance in multiple myeloma through mitochondrial functional reprogramming.
BMB Rep
September 2025
Basic Research Laboratory, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Core Research Center, Inje University, Busan 47392, Korea; Department of Health Science and Technology, College of Medicine, Inje University, Busan 47392, K
Patients with multiple myeloma develop resistance to thalidomide during therapy, and the mechanisms to counteract thalidomide resistance remain elusive. Here, we explored the interaction between cereblon and mitochondrial function to mitigate thalidomide resistance in multiple myeloma. Measurements of cell viability, ATP production, mitochondrial membrane potential, mitochondrial ROS, and protein expression via western blotting were conducted in vitro using KSM20 and KMS26 cells to assess the impact of thalidomide on multiple myeloma.
View Article and Find Full Text PDFMolecular characterization of endosomal self RNA Rmrp-engaged TLR3 dimerization to prime innate activation.
Cell Res
September 2025
Department of Immunology, Center for Immunotherapy, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
The pre-dimerization of endosome-localized RNA sensor Toll-like receptor 3 (TLR3) is required for its innate recognition, yet how TLR3 pre-dimers are formed and precisely primed for innate activation remains unclear. Here, we demonstrate that endosome-localized self RNA Rmrp directly binds to TLR3 and induces TLR3 dimerization in the early endosome but does not interact with endosome-localized TLR7, TLR8, TLR9 or cytoplasmic RNA sensor RIG-I under homeostatic conditions. Cryo-EM structure of Rmrp-TLR3 complex reveals a novel lapped conformation of TLR3 dimer engaged by Rmrp, which is distinct from the activation mechanism by dsRNA and the specific structural feature at the 3'-end of Rmrp is critical for its functional interaction with TLR3.
View Article and Find Full Text PDF