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Grassland sheep counting is essential for both animal husbandry and ecological balance. Accurate population statistics help optimize livestock management and sustain grassland ecosystems. However, traditional counting methods are time-consuming and costly, especially for dense sheep herds. Computer vision offers a cost-effective and labor-efficient alternative, but existing methods still face challenges. Object detection-based counting often leads to overcounts or missed detections, while instance segmentation requires extensive annotation efforts. To better align with the practical task of counting sheep on grasslands, we collected the Sheep1500 UAV Dataset using drones in real-world settings. The varying flight altitudes, diverse scenes, and different density levels captured by the drones endow our dataset with a high degree of diversity. To address the challenge of inaccurate counting caused by background object interference in this dataset, we propose a dual-branch multi-level attention network based on density map regression. DASNet is built on a modified VGG-19 architecture, where a dual-branch structure is employed to integrate both shallow and deep features. A Conv Convolutional Block Attention Layer (CCBL) is incorporated into the network to more effectively focus on sheep regions, alongside a Multi-Level Attention Module (MAM) in the deep feature branch. The MAM, consisting of three Light Channel and Pixel Attention Modules (LCPM), is designed to refine feature representation at both the channel and pixel levels, improving the accuracy of density map generation for sheep counting. In addition, a residual structure connects each module, facilitating feature fusion across different levels and offering increased flexibility in handling diverse information. The LCPM leverages the advantages of attention mechanisms to more effectively extract multi-scale global features of the sheep regions, thereby helping the network to reduce the loss of deep feature information. Experiments conducted on our Sheep1500 UAV Dataset have demonstrated that DASNet significantly outperforms the baseline MAN network, with a Mean Absolute Error (MAE) of 3.95 and a Mean Squared Error (MSE) of 4.87, compared to the baseline's MAE of 5.39 and MSE of 6.50. DASNet is shown to be effective in handling challenging scenarios, such as dense flocks and background noise, due to its dual-branch feature enhancement and global multi-level feature fusion. DASNet has shown promising results in accuracy and computational efficiency, making it an ideal solution for practical sheep counting in precision agriculture.
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http://dx.doi.org/10.1038/s41598-025-97929-w | DOI Listing |
PeerJ
September 2025
Department of Field Crops/Faculty of Agriculture, Çanakkale Onsekiz Mart University, Canakkale, Center, Turkey.
Context: Sustainable livestock production depends on efficient pasture management and the continuous monitoring of the health of grazing animals.
Objectives: This study investigated the effects of pasture types and sheep production systems on the hematological traits of Karacabey Merino (German Mutton Merino × Kıvırcık) ewes and lambs grazing on different pasture types throughout the year and reared in a semi-intensive system (control group).
Methods: In this twenty-six-month study, the hematological characteristics of ewes and lambs grazing on natural pastures and in spring (triticale and oat grass pasture), summer (sorghum Sudangrass and wheat stubble pasture), and autumn (triticale and oat grass pasture) were compared with ewes and lambs reared in a semi-intensive system (no pasture for lambs).
J Matern Fetal Neonatal Med
December 2025
The Fetal Institute Foundation, Miami, Miami, FL, USA.
Objective: To assess the effect of an iatrogenic gastroschisis on fetal pulmonary growth in an animal model of congenital left-sided diaphragmatic hernia (CDH).
Methods: Eighteen time-dated ewes were studied: Group I: healthy controls (H); Group II: CDH controls (untreated) (CDH); Group III: CDH and iatrogenic gastroschisis (CDH+GS). CDH was created in Groups II and III at a mean gestational age of 85 days as previously described.
Homeopathy
August 2025
Scientific Research, Centro de Pesquisa e Desenvolvimento Genética e Biotecnologia, Instituto de Zootecnia, Nova Odessa, SP, Brazil.
Gastrointestinal worm infestation, mainly by , represents one of the main health challenges in sheep farming, exacerbated by resistance to conventional anthelmintics. This study evaluated the efficacy of two homeopathic complex formulations in reducing parasite load in ewes (not lactating/not pregnant) over a period of 45 days.Thirty Santa Inês and Dorper crossbred sheep were used, distributed in three treatments: T1 ( 15cH + 11cH + 9cH + 15cH + 9cH + 6cH), T2 (the constituents of T1 but replacing by 6cH) and T3 (control group, without homeopathic treatment).
View Article and Find Full Text PDFMeat Sci
August 2025
Centre for Red Meat and Sheep Development, NSW Department of Primary Industries and Regional Development, Cowra, New South Wales, 2695, Australia.
This study investigated the use Raman spectroscopy to predict the in-pack total viable count (TVC) of microorganism on vacuum packaged and chilled lamb meat. A total of 159 lamb longissimus lumborum muscles (LL) were sourced from an investigation into the effects of chilled storage periods and packaging types. Each LL was measured while still in its packaging using a hand-held Raman spectroscopy device (785 Mira, Metrohm®, Melbourne, AUS) using a 785 ± 0.
View Article and Find Full Text PDFPLoS Negl Trop Dis
August 2025
USDA-ARS, Animal Parasitic Diseases Laboratory, Beltsville, Maryland, United States of America.
The roundworm Trichostrongylus colubriformis is one of the most important parasites in sheep, impairing feed conversion and reducing growth. However, the molecular mechanism of host resistance to this important species remains elusive. In this study, we compared gene interaction networks manifested in the jejunum transcriptome between sheep bred for parasite resistance (RES) and susceptibility (SUS) in response to a T.
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