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Article Abstract
Conventional photoelectrochemical (PEC) sensors with a single recognition interface frequently exhibit limitations in delivering comprehensive concentration information for target analytes in multiplex detection systems. The present work leverages the triple allostery of single-stranded DNA conformation to construct a "signal-on" and "signal-off" switchable PEC sensing platform, thereby propelling the advancement of DNA logic gate-integrated PEC analytical systems toward precise quantification and multiplex qualitative analysis of viruses in aquatic environments. The engineered OR-type logic gate-integrated PEC platform, harnessing the dual metal ion enzyme-mediated cascade signal amplification system, exhibited superior quantitative performance for dual-virus detection with a wider linear range from 0.01 to 100 nM and a low detection limit of 69 fM for () and 84 fM for (). Practical validation studies demonstrated that the AND-type logic gate-empowered PEC system displays an accurate qualitative capability for multiple target viruses, thus highlighting its potential for complex environmental monitoring. This work, based on the signal probe conformational allosteric-assisted DNA logic gate-enabled PEC multiplex detection, is expected to provide novel insights into the transduction mechanisms of multiplex detection signals and offer new perspectives for advanced multiplex PEC sensor development.
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| http://dx.doi.org/10.1021/acs.analchem.5c02057 | DOI Listing |
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