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To enhance the sand particle migration capability in shale oil well progressive cavity pumps, it is essential to analyze the particle migration patterns in perforated well sections. A coupled Computational Fluid Dynamics and Discrete Element Method was employed to numerically simulate sand flow characteristics in wellbores, investigating the impacts of water content, flow rate, particle size, and pump tail pipe depth on particle migration. The study revealed sand migration patterns and established a full-scale experimental setup for sub-pump particle migration, conducting solid-liquid two-phase flow experiments to examine engineering impacts of pump tail pipe depth. Results indicate: After radial inflow into the wellbore from perforations, fluid converges with underlying flow causing intense collisions that force sand-liquid mixtures into lower velocity zones, inducing particle sedimentation. As water content increases, produced fluid viscosity decreases, resulting in a sedimentation ratio that initially grows slowly before sharply rising; As the production rate increases, the sedimentation ratio gradually decreases. When the production rate exceeds 50 m/d, the sedimentation ratio stabilizes. The study further clarifies the engineering implications of positioning the pump tail pipe below the perforation interval: when the water content exceeds 70% and production drops to 30 m/d, sand production reaches 16% with a sedimentation ratio of 44.2%. If the pump tail pipe is positioned at perforated layers 7-10, the risk of sand burial becomes extremely high. Based on sand production patterns in sand-prone wells, controlling the pump tail pipe depth within perforated layers 4-7 can effectively reduce sand particle deposition, thereby mitigating hazards such as reservoir sand burial and tubing sand blockage.
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http://dx.doi.org/10.1038/s41598-025-03470-1 | DOI Listing |
Biochemistry
September 2025
Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
The recent discovery that the model multidrug efflux pump from , EmrE, can perform multiple types of transport suggests that this may be a compelling target for therapeutic intervention. Initial studies have identified several small-molecule substrates capable of inducing transporter-dependent susceptibility rather than the well-known antibiotic resistance phenotype. However, many questions regarding the underlying mechanism and regulation of this transporter still remain.
View Article and Find Full Text PDFMicrobiology (Reading)
September 2025
Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.
is an opportunistic fungal pathogen associated with superficial and systemic infections in humans. Azole antifungal resistance in is of clinical concern, and both oral and systemic infections can be difficult to treat due to the lack of alternative antifungal drugs. Expression of a hyperactive form of the transcription factor Tac1 is a major contributor to azole resistance in isolates resulting in the increased expression of the azole efflux pump Cdr1.
View Article and Find Full Text PDFElife
July 2025
Department of Biochemistry, University of Wisconsin-Madison, Madison, United States.
The model multi-drug efflux pump from , EmrE, can perform multiple types of transport leading to different biological outcomes, conferring resistance to some drug substrates and enhancing susceptibility to others. While transporters have traditionally been classified as antiporters, symporters, or uniporters, there is growing recognition that some transporters may exhibit mixed modalities. This raises new questions about their regulation and mechanism.
View Article and Find Full Text PDFSci Rep
July 2025
School of Petroleum Engineering, Yangtze University, Wuhan, 430100, Hubei, China.
To enhance the sand particle migration capability in shale oil well progressive cavity pumps, it is essential to analyze the particle migration patterns in perforated well sections. A coupled Computational Fluid Dynamics and Discrete Element Method was employed to numerically simulate sand flow characteristics in wellbores, investigating the impacts of water content, flow rate, particle size, and pump tail pipe depth on particle migration. The study revealed sand migration patterns and established a full-scale experimental setup for sub-pump particle migration, conducting solid-liquid two-phase flow experiments to examine engineering impacts of pump tail pipe depth.
View Article and Find Full Text PDFInt Immunopharmacol
September 2025
Department of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China. Electronic address:
Background: Hypertensive nephropathy (HN), a major complication of hypertension, is characterized by key pathological features such as renal inflammation and fibrosis. Recent studies have demonstrated that Agrin plays a significant role in tissues such as the heart and skeletal muscle. Its cleavage product, the C-terminal Agrin fragment 22 (CAF22), has been suggested as a potential new biomarker for renal insufficiency.
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