Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Biodegradable polymers have emerged as promising platforms for drug delivery. Produced by microbiomes, polyhydroxyalkanoates (PHAs) offer excellent biocompatibility, biodegradability, and environmental sustainability. In this study, we report the surface functionalization of PHA-based nanoparticles (NPs) using the SpyTag-SpyCatcher system to enhance cellular uptake. Initial conjugation with mEGFP-SpyTag enabled visualization, followed by decoration with HER2-specific Affibody-SpyCatcher and/or TAT-SpyCatcher peptides. The prepared NPs retained a diameter of <200 nm and a negatively charged surface. Affibody-functionalized NPs significantly enhanced internalization and cytotoxicity in HER2-overexpressing SK-BR-3 cells, whereas TAT-functionalized NPs promoted uptake across various cell types, independently of HER2 expression. Dual-functionalized NPs exhibited synergistic or attenuated effects based on the HER2 expression levels, highlighting the critical role of ligand composition in targeted delivery. The results of this study demonstrate that the SpyTag-SpyCatcher-mediated surface engineering of PHA NPs offers a modular and robust strategy for active targeting in nanomedicine.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196195 | PMC |
| http://dx.doi.org/10.3390/pharmaceutics17060721 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Improving the Thermostability and Catalytic Activity of DAAO by a Combinatorial Strategy Using Sequence Consensus Design and SpyTag/SpyCatcher Self-Cyclization.
J Agric Food Chem
September 2025
School of Chemical Engineering and Technology, Zhengzhou University, Zhengzhou 450001, China.
d-Amino acid oxidase from (DAAO) is valuable for pharmaceutical and chemical synthesis due to its high enantioselectivity, but its poor thermostability limits extensive application. This study proposed a synergistic strategy of "sequence consensus design coupled with structure modification" to enhance DAAO thermostability. Through homologous sequence analysis and greedy algorithm-based optimization, a triple mutant M3 (S18T/V7I/Y132F) was obtained, showing a 3.
View Article and Find Full Text PDFEngineering Protein-Peptide Interfaces via Combinatorial Mutagenesis and Mass Photometric Screening.
Biomolecules
August 2025
School of Biosciences, College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
The SpyTag-SpyCatcher system, developed by the Howarth lab, is based on splitting the CnaB2 domain from Streptococcus pyogenes into two parts: a 13-amino-acid SpyTag and a 116-amino-acid SpyCatcher. Upon incubation, they spontaneously form a covalent isopeptide bond between Asp7 (SpyTag) and Lys31 (SpyCatcher). This study explores whether the interaction specificity can be modulated by altering hydrophobic residues within the SpyCatcher binding pocket and corresponding SpyTag positions, potentially to create orthogonal SpyTag-SpyCatcher pairs.
View Article and Find Full Text PDFIntegrated self-assembling multi-enzyme display platform on Candida tropicalis surface for efficient degradation of PET waste.
J Hazard Mater
August 2025
Key Laboratory of Industrial Biotechnology, Ministry of Education, & School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:
The bioprocess of recycling polyethylene terephthalate (PET) has been extensively studied; however, challenges remain due to its process complexity, low enzymatic performance, and high costs. Here, we devised a strategy termed the Integrated Self-Assembling Multi-Enzyme Display Platform (ISA-MEDP) to directly and efficiently degrade postconsumer PET (pc-PET). We developed a novel surface display system for the unconventional and robust yeast Candida tropicalis, enabling FAST-PETase and MHETase to be functionally anchored onto the cell wall.
View Article and Find Full Text PDFPioneer: a synthetic human antibody phage display library for rapid therapeutic lead generation.
MAbs
December 2025
Bio-Rad AbD Serotec GmbH, Neuried, Germany.
Antibody phage display, a biotechnological method for selecting fully human antibodies from diverse libraries, is firmly established in antibody drug discovery due to its speed and flexibility. In this study, we introduce Pioneer, one of the largest synthetic human antibody libraries developed to date, with approximately 2.2 × 10 functional members.
View Article and Find Full Text PDFSpyRing-Mediated Cyclization of TEV Protease, Guided by AlphaFold, Improves Thermostability.
ACS Omega
August 2025
Institute of Scientific and Industrial Research (SANKEN), The University of Osaka, Ibaraki, Osaka 567-0047, Japan.
Cyclization is a promising strategy to enhance protein stability, but its applicability is often limited by structural constraints such as the distance between the N- and C-terminal regions. Here, we report the rational design and characterization of a cyclized Tobacco Etch Virus protease (cTEVp) using the SpyRing system, which enables covalent cyclization through SpyTag/SpyCatcher-mediated isopeptide bond formation. We applied this approach to a widely used engineered TEVp variant (L56V, S135G, S219V, Δ238-242) and employed AlphaFold structure prediction to optimize linker length and domain positioning.
View Article and Find Full Text PDF