Effects of the Ecdysone Receptor on the Regulation of Reproduction in .

The effects of the gene encoding the ecdysone receptor () on the reproduction of the ladybug was evaluated. transcription was measured by quantitative real-time PCR in ladybug adults reared on artificial diets with and without 20-hydroxyecdysone (20E). expression levels in 5 d old male and female ladybugs supplied with the 20E-amended artificial diet were lower than expression levels in ladybugs reared on an artificial diet lacking 20E. However, expression levels in 10 d old ladybugs supplied with the 20E artificial diet were higher than those lacking 20E supplementation. The regulatory effects of were studied in female and male ladybugs by RNA interference. expression in female ladybugs injected with -dsRNA was significantly downregulated after 5 d but remained unaffected in 10 d old females. expression levels in males microinjected with -dsRNA were significantly lower at 5 and 10 d after microinjection than -dsRNA-treated males. The ovary volume in females injected with -dsRNA at 5 d was smaller than females microinjected with -dsRNA, but volumes at 10 d were larger than in -dsRNA-treated females. The testes of males injected with -dsRNA were larger than those injected with -dsRNA at 5 d but the testes at 10 d after injection with -dsRNA were smaller than those injected with -dsRNA. When females were microinjected with -dsRNA and mated with noninjected males, egg production decreased by 34.80% for 20 days. When males were microinjected with -dsRNA and mated with noninjected females, egg production decreased by 30.38% for 20 days. Injection of female and male ladybugs with -dsRNA had no significant effect on egg hatching rates. Our results show that plays an important role in the development of reproductive organs and egg development in .