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Article Abstract
Vitellogenins (VTGs), being lipoproteins with high molecular weight, are the primary nutrient source for the embryonic development, and they are synthesized and accumulated massively in eggs during vitellogenesis. In this study, a novel vtg gene, Spvtg3, was discovered from our embryo transcriptome data of mud crab (Scylla paramamosain). This gene encodes a 2304-amino-acid protein featuring conserved vitellogenin domains (LPD_N, DUF1943, and VWD) and has a predicted molecular weight of 281.45 kDa. The Spvtg3 was exclusively expressed in the hepatopancreas and ovaries. During embryonic development, Spvtg3 expression dramatically increased in five pairs of appendages period. Liposome-mediated Spvtg3 dsRNA interference resulted in slower embryonic development, reduced hatching rate, and deformities. Transcriptome and metabolome analyses found some important differentially expressed genes (DEGs) in embryonic development signaling pathways (e.g., GAP junction, Wnt, and the MAPK signaling pathway), and some vital differential metabolites in metabolic pathways (e.g., lipid metabolism and pyrimidine metabolism). Furthermore, Spvtg3 and Lysophosphatidylethanolamine (LPE) expression levels were positively correlated with the expression of genes associated with embryonic development (sox21, eg, sox2, sox3, soxb2, foxl2-like, nr2e1, and fshr9). These results indicated that Spvtg3 plays a crucial role in mud crab embryonic development, potentially regulating VTG synthesis, transport, and metabolism.
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| http://dx.doi.org/10.1016/j.ijbiomac.2025.145394 | DOI Listing |
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