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Article Abstract
The Argonaute CSR-1 is essential for germline development in . Loss of CSR-1 leads to the down-regulation of thousands of germline-expressed genes, supporting a model in which CSR-1 "licenses" gene expression via a poorly understood mechanism. In contrast, a small subset of genes is up-regulated in mutants, including , which encodes a conserved GHKL-type ATPase. We show that is overexpressed in mutants and accumulates over CSR-1 licensed targets, coinciding with aberrant gain of H3K9me3, reduced H3K36me3, and transcriptional repression. Notably, loss of fully rescues these chromatin defects and partially restores gene expression and fertility in mutants. Conversely, ectopic overexpression of MORC-1 in the wild-type germ line is sufficient to repress CSR-1 licensed targets and severely compromise fertility. These findings support a model in which CSR-1 prevents MORC-1 overexpression and consequent misregulation of CSR-1 licensed genes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180489 | PMC |
| http://dx.doi.org/10.1126/sciadv.ado4170 | DOI Listing |
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