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Practical applications of anaerobic ammonium oxidation (anammox) are often hindered by slow recovery following operational failures. In this study, a novel "0 + 1 > 1" phenomenon was observed when using mixed inactive-active sludge as inoculum. In this context, "0" represented inactive sludge caused by excessive aeration, while "1" denoted active sludge. The mixed inactive-active sludge achieved faster recovery than either inactive or active sludge alone under identical conditions, demonstrating "0 + 1 > 1". Two distinct anammox genera were used: Candidatus Kuenenia as active sludge while Candidatus Brocadia as inactive sludge, revealing the primary mechanism was enhanced growth of anammox bacteria from active sludge rather than the reactivation of inactive sludge, which was related with increased relative gene abundances of enzymes involved in N metabolism, enhanced amino acid synthesis and quorum sensing (QS) communication. Reactivation of inactive sludge was excluded due to irreversible damage of hdh. This study presents a cost-effective strategy for accelerating anammox process recovery.
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http://dx.doi.org/10.1016/j.biortech.2025.132831 | DOI Listing |
Anal Chem
August 2025
College of Chemistry, Xinjiang University, Urumqi 830046, China.
The substituent design and photophysical property manipulation are of paramount importance in fluorescent probe design, particularly crucial for chemically inactive molecules that lack reactive functional groups, such as synthetic cannabinoids. Here, a ratiometric fluorescent probe, 2-(5-phenyl-8-(4-(trifluoromethyl)phenyl)pyrimido[4,5-]pyridazin-2-yl)phenol (DPTF), was selected and synthesized using trifluoromethyl as an electron acceptor for the sensitive and specific detection of one representative synthetic cannabinoid, JWH-018 (1-naphthyl(1-pentyl-1H-indol-3-yl)methanone). The DPTF probe, driven by intermolecular through-space charge transfer (TSCT), exhibits a theoretically calculated limit of detection (LOD) of 2.
View Article and Find Full Text PDFBioresour Technol
October 2025
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Key Laboratory of Urban Water Supply, Water Saving and
Practical applications of anaerobic ammonium oxidation (anammox) are often hindered by slow recovery following operational failures. In this study, a novel "0 + 1 > 1" phenomenon was observed when using mixed inactive-active sludge as inoculum. In this context, "0" represented inactive sludge caused by excessive aeration, while "1" denoted active sludge.
View Article and Find Full Text PDFWater Res
June 2025
PROSYS Research Center, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads Building 228A, Kgs. Lyngby 2800, Denmark.
In this paper, a set of mathematical tools are developed and assembled to quantify, predict and virtually assess NO emission mitigation strategies in partial nitritation (PN) / anammox (ANX) granular based reactors. The proposed approach is constructed upon a set of data pre-treatment methods, process simulation models, control tools (and algorithms) and key performance indicators to analyze, reproduce, and forecast the behavior of multiple operational variables within aerobic granular sludge systems. All these elements are tested on two full-scale data sets (#D1, #D2) collected over a period of four months (Sept-Dec 2023).
View Article and Find Full Text PDFEnviron Geochem Health
July 2024
Tianjin Center, China Geological Survey, Tianjin, 300170, China.
Miyun Reservoir plays a vital role as a source of drinking water for Beijing, however it grapples with nitrogen contamination issues that have been poorly understood in terms of their distribution, source, and associated health risks. This study addresses this knowledge gap by employing data on nitrate nitrogen (NO-N), chloride (Cl), dual isotopic compositions of NO (δN-NO and δO-NO) data in water ecosystems, systematically exploring the distribution, source and health risk of nitrogen contaminants in Miyun reservoir watersheds. The results showed that over the past 30 years, surface water runoff has exhibited a notable decrease and periodic fluctuations due to the combined influence of climate and anthropogenic activities, while the total nitrogen (TN) concentration in aquatic ecosystems presented an annual fluctuating upward trend.
View Article and Find Full Text PDFInt J Mol Sci
June 2024
Department of General and Analytical Chemistry, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
Sulfonamides can be effectively removed from wastewater through a photocatalytic process. However, the mineralization achieved by this method is a long-term and expensive process. The effect of shortening the photocatalytic process is the partial degradation and formation of intermediates.
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