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Article Abstract
We have formed new short telomeres in using an inducible nuclease that cuts near telomere repeats in cells that lack, cannot recruit or cannot fully activate telomerase. Sequencing these new telomeres showed that cells can divide at least 4 times with ~30 bp of non-telomeric sequence at the chromosome end in cells lacking telomerase, which contrasts with current models for the roles of terminal single-stranded telomere repeats and the telomere proteins in telomere protection and replication. Cells that cannot recruit or activate telomerase had similar results, with additional terminal truncations or telomere repeat addition.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163625 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.001622 | DOI Listing |
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