Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
D-allose is a rare hexose sugar with a variety of potential application in food, medicine and other fields. Ribose 5-phosphate isomerase（RPI） plays a pivotal role in the synthesis of D-allose. AtRpiB from Acetivibrio thermocellus can convert D-psicose to D-allose; however, improvements in its stability, optimal temperature, and conversion efficiency are necessary. This study aimed to investigate the catalytic properties, stability, and substrate-binding affinity of AtRpiB using D-psicose as the substrate. Using various strategies, 10 amino acid residues were selected to construct a library of 82 single mutants. Nine single mutants showed high conversion rates of D-allose. Furthermore, 27 double mutants were constructed by combining nine successful single mutation sites. Notably, the mutants R109W/R132Q, S39I/R109F, and S39V/R109F showed a 1.39-fold increase in enzyme activity at 40 ºC, 1.58-fold increase at 60 ºC, and 1.9-fold increase at 80 ºC, respectively. The S39V/R109F mutant converted 100 g/L D-psicose to 38.21 g/L D-allose after 18 hours at 40°C, the highest reported conversion rate for RpiB in D-allose production to date. Analysis of enzymatic characteristics, structure, and molecular dynamics simulations revealed that changes in the amino acid composition and conformational adjustments in loops 3, 9, and 10 of AtRpiB significantly affected the entry and exit of substrates and products into the active pocket, conversion efficiency, and enzyme stability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.enzmictec.2025.110671 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metabolomic and transcriptomic analyses unveil the accumulation of shikimic acid in the leaves of .
Front Plant Sci
August 2025
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou, China.
Introduction: Shikimic acid, as a critical precursor for oseltamivir synthesis in antiviral pharmaceuticals, faces escalating global demand. Although leaves have emerged as a promising natural source of shikimic acid owing to their exceptional content of this valuable compound and substantial biomass production capacity, the molecular mechanisms underlying its biosynthesis and downstream metabolic regulation in leaves remain largely unknown.
Methods: Here, the concentration of shikimic acid in 33 clones were assessed, and 1# (referred as HS) had the highest level.
Biphasic Salt Effects on Germination and Growth Linked to Carbon Fixation and Photosynthesis Gene Expression.
Int J Mol Sci
August 2025
National Wolfberry Engineering Research Center, Institute of Wolfberry Engineering Technology, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.
Since the onset of industrialization, the safety of arable land has become a pressing global concern, with soil salinization emerging as a critical threat to agricultural productivity and food security. To address this challenge, the cultivation of economically valuable salt-tolerant plants has been proposed as a viable strategy. In the study, we investigated the physiological and molecular responses of Murr.
View Article and Find Full Text PDFSoluble guanylate cyclase stimulation improves cardiac function and mitochondrial activity in a rat model of early-stage heart failure with preserved ejection fraction.
Biomed Pharmacother
August 2025
Cellular and Molecular Cardiology Research Unit, IDIS, Complexo Hospitalario Universitario de Santiago de Compostela, Área Sanitaria Santiago de Compostela e Barbanza (SERGAS), Santiago de Compostela, Spain; Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), Inst
Targeting cardiac mitochondrial dysfunction and cardiac metabolic reprogramming is critical for improving chronic heart failure (HF) treatment. While the soluble guanylate cyclase stimulator vericiguat has shown promise in treating HF with reduced ejection fraction (HFrEF), its effects on HF with preserved ejection fraction (HFpEF), particularly on myocardial bioenergetics, remain undefined. This study aimed to clarify the effects of vericiguat on cardiac function and metabolism in a preclinical model of early-stage HF.
View Article and Find Full Text PDFDirected Evolution of Class I Aldolases for Cooperative Photobiocatalytic Enantioselective Radical α-Alkylation of Aldehydes.
J Am Chem Soc
August 2025
Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, United States.
By harnessing the synergy between enzymes and photoredox catalysts, cooperative photobiocatalysis has recently emerged as a promising strategy for developing stereoselective radical reactions. While various cofactor-dependent enzymes have been repurposed, the use of cofactor-independent enzymes in such cooperative catalysis without requiring expensive cofactors remains rare. Herein, we report the successful repurposing of class I aldolases, a prominent family of naturally occurring, cofactor-independent enzymes, to catalyze unnatural radical α-alkylation of aldehydes in a highly enantioselective fashion.
View Article and Find Full Text PDFA Drosophila model of prion disease and its metabolic changes in the brain.
Animal Model Exp Med
July 2025
National Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Background: Prion diseases (PrDs) are fatal transmissible neurodegenerative disorders caused by misfolded prion protein, which is highly expressed in the brain. Drosophila has been employed as a model system for studying mammalian neurodegenerative diseases.
Methods: Drosophila transgenic for hamster prion protein (HaPrP) was generated by Valium20 transformation.