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Fabry-Perot cavities are essential tools for applications like precision metrology, optomechanics and quantum technologies. A major challenge is the creation of microscopic spherical mirror structures which allow the precise matching with the wavefront of a Gaussian beam, while providing high surface quality. We present a novel fabrication technique, enabling the creation of customized microscopic cavity mirror structures over a wide range of geometrical parameters, by combining focused ion beam (FIB) milling and CO laser smoothing. While FIB milling allows us to imprint features on the mirror substrate with a resolution on the nanometer scale, the application of defocused CO laser pulses consistently reduces remaining surface deformations down to a roughness of = 0.2 nm. The average deviation of the profile from a spherical shape is kept below a few nanometers. This technique enables the customized and repeatable fabrication of low loss optics on a wide range of optical substrates, including optical fibers. Thus, Fabry-Perot cavities can be fabricated with pre-defined modal volume, high finesse and tailored ellipticity. Since the structural shape of the mirror is created by FIB milling, its pixel-by-pixel nature of the writing process can be used to create arbitrary structures with a resolution given by the FIB milling. At the same time, the surface quality is given by the CO laser smoothing processes, without degrading the FIB milled shape.
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http://dx.doi.org/10.1364/OE.557212 | DOI Listing |
Proc Natl Acad Sci U S A
September 2025
Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.
We applied micro-computed tomography, high-resolution cryo-scanning electron microscopy (SEM) combined with cathodoluminescence, and cryo-focused ion beam Milling-SEM to perform three-dimensional imaging of human atherosclerotic tissues with tens of nanometers resolution, under hydrated, near-native conditions with minimal sample processing. The same technology was applied to cultured macrophages exposed to cholesterol crystals, and the observations made on the macrophages were compared to those made on the pathological tissue. We observed that cholesterol crystal digestion and, eventually, cholesterol crystal clearance occurs in the advanced human plaques through cellular processing.
View Article and Find Full Text PDFNanoscale
September 2025
National Research University of Electronic Technology - MIET, Bld. 1, Shokin Square, Zelenograd, Moscow, 124498, Russia.
The focused ion beam (FIB) technique has been established as a powerful nanofabrication tool. The application of this technique is limited due to the implantation of beam ions into the substrate, which is difficult to overcome and can be detrimental in many cases. Herein, we report that in comparison with the commonly used smooth milling process, the amount of implanted gallium atoms in the near-surface region of fabricated structures can be significantly reduced when the ions strike a highly inclined sputtering front wall formed on the substrate surface during the edge milling process of the line-by-line beam scanning strategy.
View Article and Find Full Text PDFEMBO Rep
August 2025
Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Direct visualization of HIV-1 nuclear import through the nuclear pore complex (NPC) presents a technical challenge due to the rarity of this process. To enable systematic investigation, we developed a robust in situ system that mimics HIV-1 nuclear import in a near-native context using isolated HIV-1 virus like particles (VLP) cores and permeabilized CD4 + T lymphocyte (CEM) cells. This approach supports docking and translocation of abundant viral cores through nuclear pores into the nucleus.
View Article and Find Full Text PDFCurr Opin Struct Biol
August 2025
Research Group CryoEM Technology, Max Planck Institute of Biochemistry, Martinsried, Germany. Electronic address:
Cryo-focused ion beam instruments to produce cellular thin sections for subsequent imaging by cryo-electron tomography have become an integral part of the methodologies for in situ structural biology, enabling high-resolution imaging of biological structures in their native environment. The application of these instruments has opened windows into cells that allowed unprecedented insights into the ultrastructure of cells and more recently, small multicellular organisms and tissues. While great strides have been made in the characterization of cryo-FIB milling and the streamlining of workflows with these tools, many limitations and technical challenges remain to be resolved.
View Article and Find Full Text PDFACS Sens
August 2025
Neuroelectronics, Munich Institute of Biomedical Engineering, School of Computation, Information and Technology, Technical University of Munich, Hans-Piloty-Str. 1, 85748 Garching, Germany.
Understanding the functional connectivity and behavior of 3D cell cultures and organoids requires monitoring electrical activity across multiple planes. However, traditional planar microelectrode arrays (MEAs) are limited to surface recordings and struggle to capture signals from deeper layers. Additionally, current fabrication methods face challenges such as prolonged production times and limited design flexibility, which hinder the development of high-precision 3D electrode arrays and affect the quality of cell-electrode coupling.
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