Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Amyloplasts, non-photosynthetic plastids specialized for starch synthesis and storage, proliferate in storage tissue cells of plants. To date, studies of amyloplast replication in roots and the ovule nucelli from various plant species have been performed using electron and fluorescence microscopy. However, a complete understanding of amyloplast replication remains unclear due to the absence of experimental systems capable of tracking their morphology and behavior in living cells. Recently, we demonstrated that ovule integument could provide a platform for live-cell imaging of amyloplast replication. This system enables precise analysis of amyloplast number and shape, including the behavior of stroma-filled tubules (stromules), during proplastid-to-amyloplast development in post-mitotic cells. Here, we provide technical guidelines for observing and quantifying amyloplasts using conventional fluorescence microscopy in wild-type and several plastid-division mutants of . Key features • Novel approach for investigating amyloplast differentiation and replication in plant cells. • Detection of stroma-labeled amyloplasts in whole-mount ovules using conventional fluorescence microscopy. • Facilitates quantitative and comparative analysis of amyloplast proliferation using various resources. • Enables high-resolution analysis of changing amyloplast and stromule morphologies in living cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152115 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.5333 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
RepA Protein of Citrus Chlorotic Dwarf-Associated Virus Impairs Perinuclear Chloroplast Clustering Induced by Lemon Chloroplast Malate Dehydrogenase.
Mol Plant Pathol
August 2025
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing, China.
Replication-related protein A (RepA), encoded by the citrus chlorotic dwarf-associated virus (CCDaV), induces hypersensitive response (HR)-like cell death and defence responses. However, the interactions between the host plant and CCDaV-RepA remain unclear. In this study, Citrus limon chloroplast malate dehydrogenase (ClMDH) was found to interact with CCDaV-RepA in the nucleus.
View Article and Find Full Text PDFA chaperonin complex regulates organelle proteostasis in malaria parasites.
PLoS Pathog
July 2025
Department of Microbiology and Molecular Genetics, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
The apicoplast of Plasmodium parasites serves as a metabolic hub that synthesize essential biomolecules. Like other endosymbiotic organelles, 90% of the apicoplast proteome is encoded by the cell nucleus and transported to the organelle. Evidence suggests that the apicoplast has minimal control over the synthesis of its proteome and therefore it is unclear how organelle proteostasis is regulated.
View Article and Find Full Text PDFPEN1 catalyses RNA primer removal during plastid DNA replication in maize.
Nat Plants
July 2025
State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai, China.
The plastid DNA (ptDNA) replication is initiated by primases, which synthesize RNA primers; following the synthesis of DNA fragments, primers must be removed before ligation. However, the enzymes and mechanisms underlying this process are poorly understood. Here we cloned a gene from maize that encodes a plastid-localized and Mn-dependent 5'-3' exonuclease (designated PEN1) responsible for this process.
View Article and Find Full Text PDFEnhanced chloroplast FtsZ-ring constriction by the ARC6-ARC3 module in .
Proc Natl Acad Sci U S A
July 2025
Department of Plant Sciences, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Chloroplast division, a process tightly linked to the energy demands of plants, is initiated by the formation of the stromal filamenting temperature-sensitive Z (FtsZ) ring. The Z ring is highly dynamic, and its constriction provides the essential force for chloroplast division. However, the regulatory mechanisms governing Z-ring dynamics and constriction remain poorly understood.
View Article and Find Full Text PDFUsing a Live Analysis System to Study Amyloplast Replication in Ovule Integuments.
Bio Protoc
June 2025
Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru, Nishihara, Okinawa, Japan.
Amyloplasts, non-photosynthetic plastids specialized for starch synthesis and storage, proliferate in storage tissue cells of plants. To date, studies of amyloplast replication in roots and the ovule nucelli from various plant species have been performed using electron and fluorescence microscopy. However, a complete understanding of amyloplast replication remains unclear due to the absence of experimental systems capable of tracking their morphology and behavior in living cells.
View Article and Find Full Text PDF