Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Replication-related protein A (RepA), encoded by the citrus chlorotic dwarf-associated virus (CCDaV), induces hypersensitive response (HR)-like cell death and defence responses. However, the interactions between the host plant and CCDaV-RepA remain unclear. In this study, Citrus limon chloroplast malate dehydrogenase (ClMDH) was found to interact with CCDaV-RepA in the nucleus. ClMDH induces perinuclear chloroplast clustering (PCC). Moreover, ClMDH suppressed HR-like cell death and the accumulation of reactive oxygen species (ROS) induced by CCDaV-RepA, and promoted the accumulation of CCDaV-RepA. In addition, CCDaV-RepA overexpression altered the subcellular localisation of ClMDH from the chloroplast to the nucleus and inhibited ClMDH-induced PCC. These results reflected the involvement of ClMDH-induced PCC in the host response to CCDaV infection and provide new insights into the interaction between the host and CCDaV.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12330936 | PMC |
| http://dx.doi.org/10.1111/mpp.70133 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Seed Transmission of Apple Stem Grooving Virus and Citrus Concave Gum-Associated Virus in Apple ( × ).
Phytopathology
August 2025
Cornell University AgriTech, Plant Pathology and Plant-Microbe Biology, Geneva, New York, United States;
Viruses of cultivated apple ( × ) are believed to be transmitted nearly exclusively via vegetative propagation, with few known biotic vectors and limited evidence of vertical transmission. To evaluate the seed transmission capabilities of six viruses and one viroid of apple, a large-scale seedling grow-out experiment was conducted using seeds harvested from 51 trees infected by several combinations of six viruses and one viroid. Virus detection via multiplex PCR-based amplicon sequencing followed by RT-qPCR validation demonstrated that citrus concave gum-associated virus and apple stem grooving virus were transmitted to seedlings at rates of 4.
View Article and Find Full Text PDFRepA Protein of Citrus Chlorotic Dwarf-Associated Virus Impairs Perinuclear Chloroplast Clustering Induced by Lemon Chloroplast Malate Dehydrogenase.
Mol Plant Pathol
August 2025
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing, China.
Replication-related protein A (RepA), encoded by the citrus chlorotic dwarf-associated virus (CCDaV), induces hypersensitive response (HR)-like cell death and defence responses. However, the interactions between the host plant and CCDaV-RepA remain unclear. In this study, Citrus limon chloroplast malate dehydrogenase (ClMDH) was found to interact with CCDaV-RepA in the nucleus.
View Article and Find Full Text PDFApples (), one of Iran's oldest cultivated fruit crops, hold considerable economic importance. In this study, 170 apple leaf samples representing various commercial cultivars were collected across the country. RT-PCR screening targeted five common apple-infecting viruses and two viroids: apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple green crinkle-associated virus (AGCaV), apple mosaic virus (ApMV), apple scar skin viroid (ASSVd), and hop stunt viroid (HSVd).
View Article and Find Full Text PDFDetection and In Vivo Validation of e-Probes in Meta-Transcriptomic Data via Microbe Finder (MiFi) Discovers a Novel Host and a Possible New Strain of Orchid Fleck Virus.
Viruses
March 2025
Institute for Biosecurity and Microbial Forensics, Oklahoma State University, Stillwater, OK 74078, USA.
is a recently accepted plant virus genus within the family . Species assigned to the genus consist of bi-segmented, negative sense, single-stranded RNA viruses and are transmitted by spp. Currently, there are five recognized species and two unclassified members in the genus Four out of seven-orchid fleck virus (OFV), citrus leprosis virus N, citrus chlorotic spot virus, and citrus bright spot virus-can infect citrus and produce leprosis disease-like symptoms.
View Article and Find Full Text PDFMolecular and biological characterization of infectious full-length cDNA clones of two viruses in Paris yunnanensis, including a novel potyvirus.
Sci Rep
January 2025
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Paris yunnanensis, also named as Rhizoma Paridis in the Chinese Pharmacopeia, is a perennial Chinese medicinal herb commonly grown in Southwest China. However, several viruses have been found infecting this plant in recent years. Using high-throughput sequencing (HTS) and Sanger sequencing, this study obtained the complete genome sequences of three capillovirus isolates and one potyvirus isolate.
View Article and Find Full Text PDF