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Background: Well-conducted qualitative evidence syntheses (QESs) can provide invaluable insights into complex phenomena. However, the development of an in-depth understanding depends on the analysis of rich, thick data from the included primary qualitative studies. Sampling may be needed if there are too many eligible studies. Data richness and thickness are among several criteria that can be taken into consideration when sampling studies for inclusion. However, existing tools do not address explicitly the assessment of both data richness and thickness in the context of QES.
Methods: To address this gap, we have developed, piloted, and conducted initial user testing of a richness and thickness assessment tool. The tool has been in development since 2014. Three pilot versions from three review teams have been used in six Cochrane reviews. Key members from the original three review teams subsequently came together to create a consensus-based definitive version 1 of the tool. Four review authors piloted the version 1 tool, which has been subject to initial user testing. The version 1 assessment tool consists of two components: assessing the thickness of contextual data and assessing the richness of conceptual data. The accompanying guidance emphasizes the importance of assessing data that addresses the review question.
Results: The paper provides guidance on how to apply the tool, emphasizing the importance of reaching a consensus among review authors and fostering a shared understanding of what constitutes rich and thick data in the context of the review. The potential challenges related to the time and resource constraints of this additional review process are acknowledged.
Conclusion: Version 1 of the tool represents a significant development in QES methodology, filling a critical gap and enhancing the transparency and rigor of the sampling process. The authors invite feedback from the research community to further test, refine and improve this tool based on wider user experiences.
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http://dx.doi.org/10.1002/cesm.12059 | DOI Listing |
Int J Mol Sci
August 2025
Centro de Investigación en Inmunología y Dermatología (CIINDE), Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Mexico.
Ulcerative colitis is a chronic inflammatory bowel disease characterized by persistent inflammation, immune dysregulation, gut microbiota alterations, and impaired epithelial barrier function. is a legume rich in galactooligosaccharides (GOS) that functions as a prebiotic capable of modulating the gut microbiota and mitigating ulcerative colitis-related damage. This study aimed to elucidate the effect of GOS on gut microbiota modulation and the molecular mechanisms involved in epithelial restoration and inflammation reduction.
View Article and Find Full Text PDFDent J (Basel)
August 2025
Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China.
: This study aimed to investigate the effects of the quorum-quenching enzyme -acyl-homoserine lactone (AHL)-lactonase Est816 on biofilm formation in subgingival plaque microbiota from participants with advanced periodontitis. : Subgingival plaque samples were collected from 30 adults with untreated Stage III or higher periodontitis and cultured anaerobically. Est816 was applied in vitro, with phosphate-buffered saline (PBS) serving as the control.
View Article and Find Full Text PDFJ Dent Res
July 2025
Section for Oral Ecology, Cariology, Department of Dentistry and Oral Health, Aarhus University, Aarhus C, Denmark.
The extracellular matrix has been identified as an essential determinant of virulence of dental biofilms. Therefore, enzymes that degrade the matrix are a promising preventive approach for biofilm control. Laboratory studies have consistently reported significant effects of enzyme treatment on biofilm formation, but information from randomized clinical trials (RCTs) is limited.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
July 2025
Department of Environmental Studies, Dartmouth College, Hanover, NH 03755.
Climate-driven variation in traits is crucial for predicting ecological responses to environmental change, yet global patterns and drivers of microbial trait variation remain poorly understood. Using global datasets of arbuscular mycorrhizal (AM) fungal observations linked to spore morphological traits, we show that climate shapes spore trait variation and functional diversity. Larger spore volumes were more prevalent in warm, wet climates but were associated with smaller species range sizes, suggesting a trade-off between persistence and dispersal potential.
View Article and Find Full Text PDFJ Anim Sci
January 2025
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Mineral elements are crucial for biological functions, with meat serving as a key dietary source. Despite advances in ionome analysis, the genetic mechanisms regulating mineral accumulation in meat remain poorly understood. Here, we analyze the ionome of 376 breast muscles from the large gradient consanguinity segregating population generated by Pekin duck × Liancheng white duck crosses, quantifying 7 essential mineral elements (potassium (K), phosphorus (P), sodium (Na), magnesium (Mg), calcium (Ca), iron (Fe), and zinc (Zn)).
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