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A sensitive and selective fluorescence sensor for rutin was successfully developed. The sensor was fabricated from highly fluorescent nitrogen-boron co-doped carbon quantum dots (N,B-CDs) coated with mesoporous silica molecularly imprinted polymer (MIP). Rutin was detected by this sensor (N,B-CDs @ MIP) with a linear range of 0.08-30 μM and a detection limit as low as 0.05 μM, under optimal conditions. Meanwhile, the usefulness of the developed sensor was evaluated in samples of lake water, orange, and honey with satisfactory recoveries.
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http://dx.doi.org/10.1007/s00604-025-07210-0 | DOI Listing |
Talanta
September 2025
Karamanoglu Mehmetbey University, Kamil Ozdag Science Faculty, Department of Chemistry, Karaman, 70100, Turkey.
Biogenic amines (BAs) are organic nitrogen compounds formed through microbial decarboxylation of amino acids during food spoilage and biological metabolism. Therefore, the development of rapid, selective, and cost-effective detection strategies for BAs is significant for ensuring food safety and quality. In this study, a new dicyanoisophorone-based fluorescent probe (IPC) was developed, capable of fluorescence detection of aliphatic primary amines (e.
View Article and Find Full Text PDFAnal Bioanal Chem
September 2025
Hebei Key Laboratory of Public Health Safety, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Public Health, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, China.
This work presents the development of a highly sensitive, selective, and efficient aptamer-based fluorescent sensor for detecting cortisol in human urine. Carbon quantum dots-nucleic acid aptamer (CQDs-Apt) synthesized with excellent photoluminescent properties and stability, were selected as the fluorescent probe. In the presence of MoS-NSs, CQDs-Apt adsorbed onto the surface of MoS-NSs via electrostatic and π-π interactions, leading to strong and rapid fluorescence quenching due to static quenching mechanism between them.
View Article and Find Full Text PDFAnal Methods
September 2025
State Key Laboratory of Advanced Papermaking and Paper-based Materials, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
Current detection methods for often suffer from lengthy procedures, significant technical limitations, high probe costs, and poor long-term storage stability. Herein, an "on-off-on" fluorescent probe is developed based on mannose-lectin recognition for the rapid and quantitative detection of . The probe utilizes mannose-grafted carbon dots (g-CDs-M), which specifically recognize through interaction with lectins on its surface.
View Article and Find Full Text PDFChemistry
September 2025
Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, P. R. China.
The long-term visualization of intracellular Fe dynamics and lysosomal activity is crucial for investigating the physiological roles and functions of lysosomes during the growth of organisms. The lysosome-targeted fluorescent probe (RBH-EdC), derived from rhodamine-nucleoside conjugates, demonstrates a sophisticated dual-activation design: one is Fe⁺ response, triggering spirolactam ring-opening to form xanthine structures, resulting in ≥ 1000-fold fluorescence enhancement with visible colorimetric transition (colorless→pink). Another is pH sensitivity, demonstrating protonation-dependent fluorescence amplification at the dC at site N3 (pK= 2.
View Article and Find Full Text PDFMikrochim Acta
September 2025
Department of Analytical Chemistry, China Pharmaceutical University, 24 TongJiaXiang, Nanjing, 210009, Jiangsu, China.
A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O) from hydrogen peroxide (HO), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3',5,5'-tetramethylbenzidine (TMB).
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