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Article Abstract
Atomic force microscopy (AFM) is extensively applied to measure cell and tissue mechanics but lacks a standardized organoid stiffness assessment. Here, we present a protocol for quantifying the Young's modulus of organoids via AFM, combining force-curve analysis with an optimized probe. We describe steps for preparing organoids, OCT embedding, slicing, AFM detection, and force-curve analysis. By mechanically addressing gaps in organoids, this protocol improves reproducibility and expands the capabilities of biomechanical research.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148381 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2025.103825 | DOI Listing |
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