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(MTB) remains a global health issue and continues to rank among the leading causes of death from infectious diseases worldwide. Its persistence is primarily attributed to the microorganism's challenging and time-consuming diagnosis and treatment, which drives the need for new diagnostic tests. The development of rapid, highly sensitive point-of-care (POC) tests is crucial, as these tests address the limitations of traditional methods, which are lengthy and exhibit low sensitivity. Early and rapid diagnostic tests ensure timely diagnoses and treatments for individuals while playing a pivotal role in preventing the spread of MTB and curbing societal transmission. These diagnostic tests significantly impact TB diagnosis and treatment, potentially breaking the chain of transmission and presenting a promising step toward combating the infection. Rapid and accurate diagnostic tests for MTB detection continue to attract significant attention in the literature and show promise for widespread application. However, they face challenges such as limited accessibility and usability, particularly in underdeveloped countries. The implementation of rapid tests requires careful consideration of time and resource efficiency compared with traditional tests. This study reviews the diagnostic tests developed for MTB detection, tracing their evolution from the past to the present.
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http://dx.doi.org/10.1002/smmd.70007 | DOI Listing |
Curr Opin Infect Dis
September 2025
Department of Microbiology, Royal Melbourne Hospital.
Purpose Of Review: Diagnostic stewardship (DS) aims to optimise the use of laboratory testing to improve patient care while reducing unnecessary tests. This review examines recent evidence on DS interventions to optimise the use of resources, focusing on three key areas: reducing unnecessary testing, maximising the impact of existing tests, and avoiding the overdiagnosis of hospital-acquired infections.
Recent Findings: Multiple interventions have demonstrated effectiveness in reducing unnecessary blood and urine culture testing, including clinical decision support tools, education programs, and multidisciplinary approaches.
Cell Mol Biol (Noisy-le-grand)
September 2025
Department of Public Health, College of Applied Medical Sciences, Qassim University, Buraydah, 51452 P.O. Box 6666, Saudi Arabia.
Foodborne illnesses pose a significant public health threat globally, particularly in Saudi Arabia, where the rapid growth of the food service sector has increased the risk of exposure to multidrug-resistant (MDR) bacteria. Traditional microbiological methods are often time-consuming and may lack precision, highlighting the need for faster and more accurate diagnostic alternatives. In this study, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) was employed for the rapid and precise identification of bacterial contaminants in ready-to-eat (RTE) foods, alongside an assessment of their antibiotic resistance profiles.
View Article and Find Full Text PDFLangmuir
September 2025
Centre for Biomedical Engineering, Indian Institute of Technology, Delhi, New Delhi 110016, India.
The study addresses the critical issue of sepsis diagnosis, a life-threatening condition triggered by the body's immune response to infection that leads to mortality. Current diagnostic methods rely on the time-consuming assessment of multiple biomarkers by a series of tests, leading to delayed treatment. Here, we report a platform for developing a point-of-care (POC) device utilizing electrochemical immunosensors for the dual and rapid detection of sepsis biomarkers: Procalcitonin (PCT), Interleukin-6 (IL-6), and C-reactive protein (CRP) as host markers and lipopolysaccharide (LPS) as a pathogen marker.
View Article and Find Full Text PDFJ Child Neurol
September 2025
Department of Epidemiology and Environmental Health, State University of New York at Buffalo, Buffalo, NY, USA.
Mass psychogenic illness (MPI), also known as mass sociogenic illness, is a functional neurologic symptom disorder affecting multiple people simultaneously. This study presents a pediatric MPI outbreak involving abrupt-onset tics in LeRoy, NY, during 2011-2012. The analysis provides diagnostic evidence and highlights challenges with diagnosing MPI.
View Article and Find Full Text PDFEar Hear
September 2025
Department of Otolaryngology, Head and Neck Surgery, Kyushu University, Fukuoka, Japan.
Objectives: This study aimed to investigate the potential contribution of subtle peripheral auditory dysfunction to listening difficulties (LiD) using a threshold-equalizing noise (TEN) test and distortion-product otoacoustic emissions (DPOAE). We hypothesized that a subset of patients with LiD have undetectable peripheral auditory dysfunction.
Design: This case-control study included 61 patients (12 to 53 years old; male/female, 18/43) in the LiD group and 22 volunteers (12 to 59 years old; male/female, 10/12) in the control group.