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Article Abstract
ORS3257 is an efficient symbiotic strain for and but fails with due to an effector-triggered immunity response mediated by the nodulation outer protein P2 (NopP2). To understand this incompatibility, we identified NopP2 interacting proteins in cv. KPS1, including enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), monodehydroascorbate reductase (MDHAR), and serine hydroxymethyltransferase (SHMT) as targets. Protein-protein interaction assays confirmed that NopP2 binds to these enzymes, and further analysis revealed their co-localization on the plasma membrane. Comparative transcriptomic analysis revealed NopP2 stimulates genes related to plant defense response (, , , and ), hydrogen peroxide (, , and ), and cell wall lignification (). NopP2 did not alter the expression of genes encoding the target enzymes but interfered with MDHAR activity, leading to high HO accumulation in roots. These findings suggest that NopP2 contributes to symbiotic incompatibility in by inducing a multifaceted defense response and initiating cell wall lignification early in infection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083990 | PMC |
| http://dx.doi.org/10.1016/j.isci.2025.112351 | DOI Listing |
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