[Metabolic Characteristics of F-FDG in Different Types of Myeloid Leukemia Cells and Tumor-Bearing Nude Mice].

Objective: To investigate the metabolic characteristics of F-fluorodeoxyglucose (F-FDG) in myeloid leukemia by in vitro culture of myeloid leukemia cells and construction of tumor-bearing nude mouse model.

Methods: U937, THP-1, HL60 and K562 cells were cultured . The cells in logarithmic growth phase (l×10 cells/well) were added with F-FDG, and the uptake rate of F-FDG was measured at 15, 30, 60 and 120 min after addation, respectively. The four kinds of cells were inoculated subcutaneously into the hind limbs of nude mice to establish a tumor-bearing nude mouse model. When the tumor size was about 500 mm, F-FDG was injected through the tail vein of the mice, and positron emission tomography/computed tomography was performed at 60 min after injection. The morphology of tumor-bearing cells was observed by hematoxylin-eosin (HE) staining in serial pathological sections.

Results: After co-incubation with F-FDG, the F-FDG uptake rates of U937 cells were significantly higher than THP-1, HL60 and K562 cells at 4 time points (all <0.05), and THP-1 cells were higher than K562 cells (all <0.05). The uptake rate of F-FDG by leukemia cells was rapid in the first 60 min, then tended to be stable. Pathological analysis showed that subcutaneous inoculation of U937, THP-1, HL60 and K562 cells could successfully establish tumor-bearing nude mouse models of myeloid leukemia. The F-FDG uptake value in U937 tumor-bearing nude mice was significantly higher than THP-1, HL60 and K562 tumor-bearing nude mice (all <0.01). The F-FDG uptake values in THP-1 and HL60 tumor-bearing nude mice were significantly higher than that in K562 tumor-bearing nude mice (both <0.01).

Conclusion: The tumor-bearing nude mouse model of myeloid leukemia can be successfully constructed by subcutaneous inoculation. The F-FDG uptake rate of acute myeloid leukemia (AML) cells is higher in cells cultured in vitro and tumor-bearing nude mouse model. F-FDG may have better clinical application value for AML.