Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: [F]Fallypride PET has been used to study D2/3 receptor occupancy and density in neuropsychiatric disorders including Huntington's disease (HD) and aging in humans. Nevertheless, the various synthetic methods including those provided by commercial synthesizers for [F]fallypride exhibit a disadvantage concerning the necessity of using a HPLC purification step, which causes difficulties in the automation, leads to long synthesis times and moderate yields. Therefore utilizing the purification step by SPE cartridges is considered highly desirable for future commercialization of radiopharmaceutical cassettes. In our lab we have developed a simplified reliable automatic Radiosynthesis of [F]fallypride by using SPE cartridges for the purification step without the need of HPLC.
Results: A simplified Radiosynthesis of [F]fallypride has been developed without the use of HPLC for both a commercial cassette based synthesis system (AllinOne (AiO) system, Trasis, Belgium) and a research synthesis module with fixed tubing (RNplus, Synthra, Germany). The cleaning step involves a serial combination of several SPE cartridges. The synthesis time was shortened by 44% compared to synthesis using HPLC. At the same time the not decay corrected yield increases from 44 to 59% by using TBAHCO as phase transfer catalysts and from 17 to 31% for the synthesis with KCO/Kryptofix-[2.2.2] compared to synthesis using HPLC. The Radiochemical purity was always > 98% and all quality control parameters (e.g. sterility, endotoxin, stability and Radiochemical purity) conformed with requirements of the European Pharmacopoeia.
Conclusions: A GMP compliant automatic synthesis of [F]fallypride including purification using simple solid phase extraction cartridges instead of HPLC was developed and evaluated. The implementation of the simplified synthesis in both used commercial modules allows efficient and reproducible Radiosynthesis of [F]fallypride and leads to short synthesis times and high radiochemical yields with high radiochemical purity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050253 | PMC |
| http://dx.doi.org/10.1186/s41181-025-00343-w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A simple protocol to improve touch DNA analysis using direct STR amplification.
Sci Justice
September 2025
Department of Chemistry and Forensic Science, Eastern Kentucky University, 521 Lancaster Avenue, Richmond, KY 40475, United States. Electronic address:
Traditionally, when processing DNA samples, a multiple-step procedure is followed; after a sample has been collected, DNA is then extracted and quantified before a profile is generated. During the process, valuable DNA can be lost and/or consumed. When processing reference samples, where DNA is usually in abundance, DNA loss may not be a concern for the analysts.
View Article and Find Full Text PDFExploring green methods for extracting nitroexplosives from plant matrices: a chemometric strategy and comparison of enzymatic and conventional methods.
Talanta
September 2025
Warsaw University of Technology, Faculty of Chemistry, Chair of Analytical Chemistry, Noakowskiego St. 3, 00-664, Warsaw, Poland. Electronic address:
The contamination of agricultural soils with military-grade explosives such as 2,4,6-trinitrotoluene (TNT), 1,3,5-trinitro-1,3,5-triazaccyclohexane (RDX) and 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclohexane (HMX) is an emerging concern in post-conflict regions, where food crops may take up these compounds. This study presents a novel analytical approach for detecting explosive residues in wheat (Triticum aestivum L.) grown on contaminated substrates.
View Article and Find Full Text PDFThe hydrogenation side-reaction in copper-mediated radiofluorination.
EJNMMI Radiopharm Chem
September 2025
Department of Experimental Neurooncological Radiopharmacy, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Institute of Radiopharmaceutical Cancer Research, 04318, Leipzig, Germany.
Background: Copper-mediated radiofluorination (CMRF) is a breakthrough in F-radiochemistry, enabling F incorporation into molecules even at electron-rich aromatic positions. In recent years, several improved protocols have been reported to advance the application of CMRF. These advancements primarily focus on improving radiochemical conversion, expanding substrate scope, and enabling scalability for remote-controlled radiotracer production.
View Article and Find Full Text PDFBiotechnological Application of New Cold-Active Protease From Aeromonas salmonicida subsp. salmonicida EDT1.
Biotechnol Appl Biochem
September 2025
Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, Erzurum, Turkey.
This study involved the isolation of ten psychrophilic bacterial strains from cold water in Söğütlü village, Erzurum. Following isolation, the strains were characterized using molecular and conventional methods. On the basis of the results of Petri dish assays, Aeromonas salmonicida subsp.
View Article and Find Full Text PDFToward a Genetic Signature of Resistance to Activity-Based Anorexia in Striatal Projecting Cortical Neurons.
Int J Eat Disord
September 2025
Department of Physiology, Monash University, Clayton, Victoria, Australia.
Objective: Converging evidence from neuroimaging studies and genome-wide association study (GWAS) suggests the involvement of prefrontal cortex (PFC) and striatum dysfunction in the pathophysiology of anorexia nervosa (AN). However, identifying the causal role of circuit-specific genes in the development of the AN-like phenotype remains challenging and requires the combination of novel molecular tools and preclinical models.
Methods: We used the activity-based anorexia (ABA) rat model in combination with a novel viral-based translating ribosome affinity purification (TRAP) technique to identify transcriptional differences within a specific neural pathway that we have previously demonstrated to mediate pathological weight loss in ABA rats (i.