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To realize high-capacity holographic multiplexing, we propose an off-axis frequency-modulated continuous-wave digital holography (FMCW-DH) that utilizes both temporal and spatial frequencies as the multiplexable bandwidth. The temporal and spatial carrier frequencies for the multiplexed wavefronts are achieved by the frequency-modulated continuous-wave technique of the light source and the off-axis configuration of digital holography, respectively. The effectiveness of the proposed technique is demonstrated by multiplexing the multiple holograms generated by illuminating two objects at two wavelengths. Also, the phase distribution maps reconstructed from the recorded complex wavefronts were used to measure the surface profile of the known step height by using the two-wavelength method.
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http://dx.doi.org/10.1364/OL.558730 | DOI Listing |
Eur Heart J Cardiovasc Imaging
September 2025
Department of Diagnostic and Interventional Radiology, University Hospital Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.
Aims: Fetal circulation undergoes complex changes in congenital heart disease (CHD) that are challenging to assess with fetal echocardiography. This study aimed to assess clinical feasibility and diagnostic value of 4D flow cardiac magnetic resonance (CMR) in fetal CHD.
Methods And Results: Pregnant women in advanced third trimester pregnancy with fetal CHD were prospectively recruited for fetal CMR between 08/2021 and 11/2024.
Nat Med
September 2025
Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Immune checkpoint blockade (ICB) is standard of care in advanced diffuse pleural mesothelioma (DPM), but its role in the perioperative management of DPM is unclear. In tandem, circulating tumor DNA (ctDNA) ultra-sensitive residual disease detection has shown promise in providing a molecular readout of ICB efficacy across resectable cancers. This phase 2 trial investigated neoadjuvant nivolumab and nivolumab/ipilimumab in resectable DPM along with tumor-informed liquid biopsy residual disease assessments.
View Article and Find Full Text PDFJ Vis Exp
August 2025
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology.
We present multimodal confocal Raman micro-spectroscopy (RS) and tomographic phase microscopy (TPM) for quick morpho-chemical phenotyping of human breast cancer cells (MDA-MB-231). Leveraging the non-perturbative nature of these advanced microscopy techniques, we captured detailed morpho-molecular data from living, label-free cells in their native physiological environment. Human bias-free data processing pipelines were developed to analyze hyperspectral Raman images (spanning Raman modes from 600 cm to 1800 cm, which uniquely characterize a wide range of molecular bonds and subcellular structures), as well as morphological data from three-dimensional refractive index tomograms (providing measurements of cell volume, surface area, footprint, and sphericity at nanometer resolution, alongside dry mass and density).
View Article and Find Full Text PDFJ Neuromuscul Dis
September 2025
Department of Neurology, University of Kansas Medical Center, Kansas City, KS, USA.
Background: Electrical impedance myography (EIM) has been proposed as an efficient, non-invasive biomarker of muscle composition in facioscapulohumeral muscular dystrophy (FSHD).
Objective: We investigate whether EIM parameters are associated with muscle structure measured by magnetic resonance imaging (MRI), muscle histology, and transcriptomic analysis as well as strength at the individual leg muscle level.
Methods: We performed a multi-center cross-sectional study enrolling 33 patients with FSHD.
Front Microbiol
August 2025
BIOASTER, Lyon, France.
We propose an innovative technology to classify the Mechanism of Action (MoA) of antimicrobials and predict their novelty, called HoloMoA. Our rapid, robust, affordable and versatile tool is based on the combination of time-lapse Digital Inline Holographic Microscopy (DIHM) and Deep Learning (DL). In combination with hologram reconstruction.
View Article and Find Full Text PDF