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Article Abstract
Insulin is an important component of stem cell cultures; however, its role in the proliferation of avian primordial germ cells (PGCs) is unknown. The proliferation of PGCs in cultures varies and the growth factors and signaling pathways necessary to induce the proliferation of PGCs in chickens are unknown. Therefore, we conducted the present study to investigate the effect of insulin on the survival and proliferation of PGCs. In this study, we observed that under this culture system, PGCs proliferate in the presence of insulin, but do not proliferate in the absence of insulin. Furthermore, in insulin-lacking media, the expression of pluripotency genes, including , , , and was markedly decreased. Similarly, the expression of cell adhesion proteins , , and was significantly reduced. Elevated levels of ROS, GSSG, and MDA reduced the redox capacity of the cells and induced apoptosis. Subsequent transcriptome analyses revealed that insulin is one of the key factors in the proliferation of chicken PGCs through the regulation of downstream genes by PI3K/AKT, ECM-receptor interaction, Wnt, and P53 signaling, and that these downstream genes may be important for PGCs' proliferation and survival.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11988930 | PMC |
| http://dx.doi.org/10.3390/ijms26073122 | DOI Listing |
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