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http://dx.doi.org/10.1186/s13036-025-00501-3 | DOI Listing |
GM Crops Food
December 2025
Biosafety Division, National Institute of Agricultural Sciences, Jeonju, Korea.
Accurate quantification of genetically modified organisms (GMOs) is essential for regulatory compliance, especially under threshold-based labeling policies. In this study, we developed and validated twelve event-specific duplex chamber- or chip-based digital PCR (cdPCR) methods using microfluidic array plates to quantify GM maize events approved in South Korea. In contrast to conventional real-time PCR, the cdPCR approach allows for absolute quantification without standard curve calibration and incorporates event-specific zygosity ratio correction to improve accuracy of the measurement.
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September 2025
Department of Mechanical Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Accurate and rapid quantification of nucleic acid targets is crucial for molecular diagnostics, particularly in resource-limited settings where simple and robust technologies are required. This study presents a high-throughput digital loop-mediated isothermal amplification (dLAMP) platform for the absolute quantification of nucleic acids in a sample, using a microfluidic device comprising ten thousand nanoliter-scale reaction microchambers. The polydimethylsiloxane (PDMS)-based device achieved complete liquid compartmentalization within 60 s in a single operation using an electronic pipette, without requiring surface modification, pre-degassing, pre-priming, or external pumping systems, which are typically necessary in conventional methods.
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August 2025
Department of Biomechatronics Engineering, National Taiwan University, Taipei, Taiwan, Republic of China.
Correction for 'Pipette-operable microfluidic devices with hydrophobic valves in sequential dispensing with various liquid samples: multiplex disease assay by RT-LAMP' by Yen-Wei Chang , , 2024, , 3112-3124, https://doi.org/10.1039/D4LC00209A.
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July 2025
Department of Mechanical Engineering, University of Victoria, Victoria, BC, Canada.
[This corrects the article DOI: 10.3389/fbioe.2025.
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