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[This corrects the article DOI: 10.3389/fbioe.2025.1551199.].
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http://dx.doi.org/10.3389/fbioe.2025.1642571 | DOI Listing |
Microsc Res Tech
August 2025
Key Laboratory of Laser Life Science, Ministry of Education, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong, China.
Illumination uniformity is essential for quantitative analysis in fluorescence microscopy. However, fewer images or sparse intensity information can compromise correction quality of retrospective methods, whereas traditional prospective methods necessitate recalibration whenever imaging conditions change. In this paper, we theoretically for the first time analyze the impact of non-uniform illumination on quantitative fluorescence resonance energy transfer (FRET), which is also demonstrated experimentally.
View Article and Find Full Text PDFJ Biophotonics
August 2025
School of Optical and Electronic Information - Wuhan National Laboratory for Optoelectronics - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, China.
Single-objective light sheet fluorescence microscopy (LSFM) excels in high-speed imaging of large samples, but its limited fluorescence collection angle would degrade system resolution due to the fluorescence loss at low magnifications. To address this issue, we introduce a new method using a coaxial modulation module. By combining two sets of cylindrical lenses with the camera's rolling shutter, we achieve 3D imaging with an optimized fluorescence collection angle in low-magnification mode.
View Article and Find Full Text PDFFront Bioeng Biotechnol
July 2025
Department of Mechanical Engineering, University of Victoria, Victoria, BC, Canada.
[This corrects the article DOI: 10.3389/fbioe.2025.
View Article and Find Full Text PDFBioact Mater
October 2025
Department of Anesthesiology and Perioperative Medicine, Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's
Abnormal mitochondrial division in microglia significantly impacts central nervous system (CNS) diseases. However, treating CNS diseases through microglial mitochondria presents several challenges: intracerebral delivery of drugs, microglial targeting, and mitochondrial regulation. Herein, a novel three-stage sequential targeted nasal drops delivery system that achieves precise drug delivery to the core of brain lesions through noninvasive nasal delivery, targeting microglia, and regulating mitochondria were developed.
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