Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Transit time is a key in vivo metric of gastrointestinal (GI) motility, which is a physiologic readout of cellular communication within the enteric system. Here, we present a protocol to characterize longitudinal gut motility in mice. We describe steps for transit testing, whole-mount immunostaining, and tissue harvest. We then detail procedures for image processing and manual cell counting. This protocol seeks to minimize inter-trial variability while assessing cellular and molecular features that may underpin motility differences between experimental conditions. For complete details on the use and execution of this protocol, please refer to Frith et al..
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022682 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2025.103761 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regulation of sub-Tg solid-state surface crosslinking of reactive polyarylene ether substrate into loose nanofiltration membranes for dyes desalination.
Water Res
August 2025
School of Materials and Energy, University of Electronic Science and Technology of China, 610054 Chengdu, China; Sichuan Province Engineering Technology Research Center of Novel CN Polymeric Materials, Chengdu, China. Electronic address:
The scalable fabrication of high performance dyes desalination loose nanofiltration (LNF) membrane through facile thermal annealing remains challenging due to the susceptible pore collapse. Herein, we have developed a metal ion mediated sub-Tg thermal crosslinking protocol, which can convert the phase inverted reactive polymeric ultrafiltration substrate into LNF membrane showing high permselectivity as well as resistance to both extremely acid and alkaline solution. The original ultrafiltration substrate was composed of scalable-produced reactive polyarylene ether amidoxime (PEA) that was pre-crosslinked with ferric ions.
View Article and Find Full Text PDFBridging lab and field: Tracking environmentally relevant nanoplastics in crops using Py-GC/MS.
J Hazard Mater
August 2025
Univ. Rennes, CNRS, Géosciences Rennes - UMR 6118, F-, Rennes 35000, France.
The increasing presence of nanoplastics (NPs) in terrestrial environments raises concerns about their bioavailability and potential impacts on crops. This study investigates the uptake and translocation of environmentally relevant polystyrene nanoplastics (eNPs-PS) in Hordeum vulgare L. via soil.
View Article and Find Full Text PDFRefinement of post-acidification steps in the unified BARGE method for determination of metal bioaccessibility in consumer products: Addressing precipitation issues.
Anal Chim Acta
November 2025
Department of Civil and Environmental Engineering, Hanyang University, Ansan, 15588, Republic of Korea. Electronic address:
Background: Standard Unified BioAccessibility Research Group of Europe (BARGE) Method (UBM) protocols for metal bioaccessibility assessment face challenges due to post-acidification precipitation, causing significant methodological inconsistencies across studies. This research systematically examined precipitate formation by characterizing protein-metal interactions and identifying specific proteins involved, leading to development of an enhanced UBM method for more reliable metal bioaccessibility measurements in consumer products. We focused on precipitation caused by acid injection during sample storage for subsequent instrumental analysis.
View Article and Find Full Text PDFPreparation and characterization of IgY antibody and its application in immunochromatographic assay for bongkrekic acid and its isomer.
Anal Chim Acta
November 2025
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address:
Egg yolk immunoglobulin (IgY) has emerged as a promising alternative to monoclonal antibodies (mAbs) due to its facile extraction, higher yield, and greater tolerance to organic solvents. This work developed a selective IgY antibody against bongkrekic acid (BA) and isobongkrekic acid (IsoBA), the lethal toxins produced by Burkholderia gladioli pv. Cocovenenans (BGC), which led to severe food poisoning incidents and resulted in casualties.
View Article and Find Full Text PDFKetogenic Diets: Dietary Therapeutic Potential on Breast Cancer.
Semin Cancer Biol
September 2025
Dipartimento di Psicologia e Scienze della Salute, Università Telematica Pegaso, Centro Direzionale Isola F2, Via Porzio, 80143 Naples, Italy.
Among the various types of tumors, breast cancer (BC) has a high distribution in the world population and is responsible for a high mortality rate. Like other forms of cancer, BC is characterised by distinctive features such as high-energy metabolism in tumor cells, genetic mutations, and mitochondrial dysfunction that sometimes make conventional therapies less effective. However, there is a growing awareness of the vital role played by diet therapy in the overall management of the patient with BC, both by supporting standard therapy and by directly targeting aberrant biological processes involved in carcinogenesis.
View Article and Find Full Text PDF