Synergistic Amylase and Debranching Enzyme Catalysis to Improve the Stability of Oat Milk.

Foods

Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food Science and Engineering, Ningbo University, Ningbo 315832, China.

Published: April 2025


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Oat starch plays a crucial role in the stability of oat milk. Enzyme-hydrolyzed oat starch has been demonstrated to be an effective means of improving the stability of oat milk. The effects of different enzyme combinations on the stability of oat milk and the properties of starch in oats were investigated by adding α-amylase, amyloglucosidase, and different ratios of pullulanase and isoamylase. The results showed that as the degree of hydrolysis increased, the molecular weight, amylose content, and side chain length distribution of the starch decreased significantly. Moreover, compared with oat starch, the rheological and emulsifying properties of the starch hydrolysates were improved, and the characterization of emulsion stability showed that a 1:2 ratio of pullulanase to isoamylase promoted effective debranching and thus improved the stability of oat milk. This study demonstrated that debranching enzymes enhance the enzymatic hydrolysis of beverages and improve the physicochemical properties and stability of oat milk.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11988502PMC
http://dx.doi.org/10.3390/foods14071271DOI Listing

Publication Analysis

Top Keywords

stability oat
24
oat milk
24
oat starch
12
oat
9
properties starch
8
pullulanase isoamylase
8
stability
7
milk
6
starch
6
synergistic amylase
4

Similar Publications

Improvement of physicochemical properties and stabilization of oat milk by composite enzymatic hydrolysis.

Food Res Int

November 2025

School of Life and Health Sciences, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, Hubei 430

This study aimed to examine the impact of composite enzymatic treatment on the physicochemical properties of oat milk, which would provide an effective strategy to improve the stability of plant-based milk. Oat milks treated with individual α-amylase or in combination with the protein glutaminase were produced. The result indicated that composite enzyme treatment significantly changed the physicochemical properties and significantly improved the stability of oat milk.

View Article and Find Full Text PDF

In this study, tannic acid (TA) was applied to remodel the structure of quercetin-loaded oat globulin fibrils (UF-Que), to form novel fibril-based composite hydrogels (UF-Que-TA) to encapsulate Que. The hydrogels were prepared by varying the [TA]/[UF] ratio to investigate the impact of TA on gelation behavior, microstructure, molecular interactions, and stability of Que. Physicochemical results indicated that the incorporation of TA significantly enhanced the gel strength and promoted non-covalent interactions including hydrogen bonding, hydrophobic interactions, and ionic interactions.

View Article and Find Full Text PDF

Stay-Green Trait Enhances Grain Yield, Nutritional Quality, and Seed Germination Ability in Oat ( L.) on the Qinghai-Tibet Plateau.

Plants (Basel)

August 2025

Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, China.

Oat is a dual-purpose crop valued for both grain and forage. The stay-green (SG) trait, which delays leaf senescence and prolongs photosynthesis, has been shown to increase yield and quality in several crop species, yet its performance across diverse environments in oats remains underexplored. In this study, multi-location field trials were conducted in Ledu, Huangzhong and Haiyan, Qinghai Province, China, to comprehensively evaluate the performance of stay-green oat lines.

View Article and Find Full Text PDF

Nitrate and Perchlorate Reduction by a Dinuclear Mo(V) Complex.

Inorg Chem

August 2025

Department of Chemistry, Brown University, Providence, Rhode Island 02912, United States.

Nitrate (NO) and perchlorate (ClO) are persistent groundwater contaminants due to their high stability and solubility. Microorganisms reduce these anions using molybdenum-containing enzymes such as nitrate reductases and perchlorate reductases. Reported here is a bioinspired dinuclear Mo(V) catalyst, [MoO(L)(THF)] (), where L = 5-Bromo-2-hydroxybenzaldehyde thiosemicarbazone, and its reactivity with nitrate and perchlorate.

View Article and Find Full Text PDF

Tungsten model complexes that replicate aspects of enzymatic activity are scarce due to challenges in reducing the readily prepared WO moiety. As a result, studies of bioinspired catalytic cycles remain limited, since tungsten in nature primarily facilitates redox processes such as Oxygen Atom Transfer (OAT). In our group, we recently observed that pyridine-2-thiolate (PyS)-supported tungsten dioxido complexes can be reduced to phosphine-stabilized W(IV) compounds, opening up opportunities for investigating rarely observed reduced participants in the OAT catalytic cycles.

View Article and Find Full Text PDF